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Revision as of 18:21, 10 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • pan locus tag?: SAUPAN006349000
  • symbol: SA_RS13880
  • pan gene symbol?: phoB
  • synonym:
  • product: alkaline phosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • symbol: SA_RS13880
  • product: alkaline phosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2715011..2716435
  • length: 1425
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq: WP_000953755 NCBI

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACA
    GTTTTCAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGT
    TTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATG
    GGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATTCGCATGCCAAAGAAATG
    ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCA
    AAACAGAATATTACTGATTCTGCAGCTGGCGGAACAGCCTTTAGTTCTGGCCATAAGACA
    TATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAGTAAAGACAGTGCTTGAACGT
    GCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
    CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAA
    TTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCT
    AAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAC
    CTTGCCACGAACAGTAAGGATTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
    GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGAT
    ATGCAACAAAGTGCACTTAGTAAGCTTGAGCGTAATAAAAAAGGTTTCTTTTTAATGGTC
    GAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATATTACAGGCGTTATGTCTGAA
    ATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
    ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAA
    GGATACGAATGGAATCCTCAACCGATTAAATCGATGAAACACTCTGGATCATATATGACA
    GAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTAATGAAGGTTATGGCTTTGAA
    TTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGTTGCAAAAATTACTT
    AAAAAAGAAAAAGACGAAAAATCTGCAGATGTAGAAAAACAAACAAAAGCCTTACAACAC
    GCAATTCAGAAGCCAGTAAATGATAAATCTTATACAGGGTGGACTTCCGATGGACATACA
    GGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAGAATCATTTAGTGGTAATATC
    GAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1425

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS13880 [old locus tag: SA2420 ]
  • symbol: SA_RS13880
  • description: alkaline phosphatase
  • length: 474
  • theoretical pI: 9.5982
  • theoretical MW: 51920.9
  • GRAVY: -0.762025

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.3.1?  ExPASy
    Alkaline phosphatase A phosphate monoester + H2O = an alcohol + phosphate
  • TIGRFAM:
  • TheSEED: see SA2420
  • PFAM:
    Alk_phosphatase (CL0088) Alk_phosphatase; Alkaline phosphatase (PF00245; HMM-score: 389)
    and 4 more
    Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 24.9)
    no clan defined DUF1889; Domain of unknown function (DUF1889) (PF08986; HMM-score: 14.8)
    TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 14.1)
    NADP_Rossmann (CL0063) Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 14.1)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.991774
    • TAT(Tat/SPI): 0.003916
    • LIPO(Sec/SPII): 0.002577
    • Cleavage Site: CS pos: 30-31. AIA-QS. Pr: 0.8454
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446876499 NCBI
  • UniProt: see SA2420
  • protein Genbank : _
  • RefSeq: WP_000953755 NCBI

⊟Protein sequence[edit | edit source]

  • MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

⊟Peptides[edit | edit source]

  • experimentally validated:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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