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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12630 [old locus tag: SA2203 ]
  • pan locus tag?: SAUPAN005949000
  • symbol: SA_RS12630
  • pan gene symbol?: mdeA
  • synonym:
  • product: drug:proton antiporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12630 [old locus tag: SA2203 ]
  • symbol: SA_RS12630
  • product: drug:proton antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2471701..2473104
  • length: 1404
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2471701..2473104) NCBI
  • BioCyc: SA_RS12630 BioCyc
  • MicrobesOnline: see SA2203

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTGATGGGTGGATTTTTTGGATTATTAAATGAAACACTATTAGTGACGGCTTTACCA
    AGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTT
    TTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACA
    AGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTG
    AGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCGTTAGGCGCAGGTATT
    ATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCGCATGAACGCGGTAAA
    TATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCA
    GGTTACCTTGTTGAATATTTAAACTGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCA
    GCTGTTACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGAACTACAATTAAAGTA
    CCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGA
    ACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGA
    GGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTATCAACACCATTATTA
    AATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATTATTATGGGTGTCACA
    ATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCAT
    CGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATG
    TCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAAGCTTGCTTTAGTAGGT
    ATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGATGAACAAACATCAACA
    ATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCA
    GTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACACGGTTCATCTATG
    ACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCATTAATTACTATTTTA
    TCTCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAATGGCATGAACAAAATT
    GATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTA
    TTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGGAAGAAA
    AAAGAAGTTGATTCTCGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1404

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS12630 [old locus tag: SA2203 ]
  • symbol: SA_RS12630
  • description: drug:proton antiporter
  • length: 467
  • theoretical pI: 9.85404
  • theoretical MW: 50764.5
  • GRAVY: 0.777088

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 337.8)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 337.8)
    and 16 more
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 93.6)
    multidrug resistance protein (TIGR00880; HMM-score: 81)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 44.9)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 33.7)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 28)
    oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 26.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 20.9)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 18)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 17.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 17)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 15.8)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 7.5)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 5.2)
  • TheSEED: see SA2203
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 180.4)
    and 3 more
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 29.9)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.2)
    no clan defined MerC; MerC mercury resistance protein (PF03203; HMM-score: 6.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006867
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.010616
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]