From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07540 [old locus tag: SA1330 ]
  • pan locus tag?: SAUPAN004032000
  • symbol: SA_RS07540
  • pan gene symbol?: nudF
  • synonym:
  • product: NUDIX hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07540 [old locus tag: SA1330 ]
  • symbol: SA_RS07540
  • product: NUDIX hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1537591..1538133
  • length: 543
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1537591..1538133) NCBI
  • BioCyc: SA_RS07540 BioCyc
  • MicrobesOnline: see SA1330

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGATTTAAATGAAAAAACAATTGATCGAACAGTTATTTATAATGGCAAAATTGTAGAT
    GTAGAAATTCATACAGTGACATTACCAAACGGTGAAACGTCAACAAGAGAATTAGTTTAT
    CATAATGGTGCAGTTGCTGTTTGTGCATTGACACCTAAAAAAGAAGTCGTATTAGTGAAA
    CAGTATCGTAAACCAGTAGAAAAACCATTGCTAGAAATTCCAGCTGGTAAATTAGAAGAT
    GATGAAAATAGAGTCGAAGCGGCTAAGCGTGAATTAGAAGAAGAAACAGGATATATTGCC
    AAAGAGTTAACACATGTTGTAGATATGTATGGTTCACCAGGTTTTTGCGATGAACAATTA
    TCAATTTATTTTACTGATAATTTAGAAGAAGGTACAGTTCACTTAGATGAAGATGAATTT
    GTCGAAGTCATTAAAGTTCCGATTGAGAATGTTAAATCGATGCTAATGAATAAAGAAATA
    GAAGATGCGAAGACTATCATTGCATTGCAACACCTATTATTAAATTATAATCATTCTAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    543

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS07540 [old locus tag: SA1330 ]
  • symbol: SA_RS07540
  • description: NUDIX hydrolase
  • length: 180
  • theoretical pI: 4.50599
  • theoretical MW: 20452.2
  • GRAVY: -0.359444

Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity nudix-type nucleoside diphosphatase, YffH/AdpP family (TIGR00052; EC 3.6.1.-; HMM-score: 70.4)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair nucleoside triphosphatase YtkD (TIGR02705; EC 3.6.1.-; HMM-score: 31.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair mutator mutT protein (TIGR00586; EC 3.6.1.-; HMM-score: 21)
  • TheSEED: see SA1330
  • PFAM:
    NUDIX (CL0261) NUDIX; NUDIX domain (PF00293; HMM-score: 67.2)
    and 1 more
    no clan defined DUF3982; Protein of unknown function (DUF3982) (PF13138; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00588
    • TAT(Tat/SPI): 0.000656
    • LIPO(Sec/SPII): 0.000778
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGAVAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKELTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEIEDAKTIIALQHLLLNYNHSK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]