From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07200 [old locus tag: SA1270 ]
  • pan locus tag?: SAUPAN003891000
  • symbol: SA_RS07200
  • pan gene symbol?:
  • synonym:
  • product: amino acid permease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07200 [old locus tag: SA1270 ]
  • symbol: SA_RS07200
  • product: amino acid permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1471747..1473069
  • length: 1323
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1471747..1473069) NCBI
  • BioCyc: SA_RS07200 BioCyc
  • MicrobesOnline: see SA1270

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAAATGGTAAAGAATTACAAAAAAATATAGGTTTCTTCTCAGCGTTTGCTATTGTT
    ATGGGGACAGTTATTGGTTCAGGAGTATTCTTTAAAATATCAAACGTAACAGAAGTAACA
    GGAACAGCAGGAATGGCCTTGTTTGTATGGTTCCTAGGCGGCATCATTACCATTTGTGCG
    GGGTTAACAGCAGCAGAACTTGCTGCTGCAATCCCTGAAACAGGTGGCTTAACGAAGTAT
    ATAGAATATACATACGGTGATTTCTGGGGCTTCCTATCAGGTTGGGCGCAATCATTTATT
    TATTTTCCAGCTAACGTAGCAGCATTGTCTATCGTATTTGCGACACAGCTAATTAATTTA
    TTCCATTTATCTATAGGTTCGTTAATACCAATAGCAATCGCATCTGCGTTATCTATTGTG
    TTGATAAATTTCCTAGGTTCAAAAGCAGGCGGAATTTTACAATCAGTTACTTTAGTAATT
    AAACTGATTCCAATCATCGTTATTGTAATTTTTGGTATTTTTCAATCTGGAGATATCACT
    TTTTCATTAATTCCAACTACAGGTAATTCAGGAAATGGTTTCTTTACAGCAATTGGTAGT
    GGTTTATTAGCAACTATGTTTGCATATGATGGTTGGATTCATGTAGGAAATGTTGCGGGG
    GAACTTAAAAATCCTAAACGCGATTTACCTTTAGCGATTTCAGTTGGTATCGGTTGTATT
    ATGGCTGTGTATTTATTAATTAACGCTACATTTTTATTAACGTTGCCAATAGAACTACTT
    GCTGGTAACTTAAATGCAGCTTCAGATACATCAAAAATATTATTTGGTGAAAATGGCGGT
    AAAATTATTACAATCGGTATATTAATTTCTGTTTATGGTACGATCAATGGCTATACTATG
    ACTGGTATGCGCGTACCATATGCAATGGCTGAAAGAAAATTATTGCCATTTAGCCACTTA
    TTCGCAAAATTAACAAAATCTGGCGCACCATGGTTTGGCGCAATTATACAACTTATAATC
    GCTATCATCATGATGTCAATGGGAGCATTTGATACAATAACAAACATGTTAATCTTTGTT
    ATTTGGTTGTTCTATTGTATGTCATTTGTTGCTGTAATAATTTTAAGAAAACGTGAACCA
    AATATGGAGCGTCCATATAAAGTACCGTTATATCCGATCATACCTTTAATTGCTATTTTG
    GCAGGATCATTTGTATTGATTAATACACTGTTTACACAATTTATATTAGCAATCATTGGA
    ATTCTAATAACAGCACTTGGTATACCAGTTTATTACTATAAGAAGAAACAAAAAGCAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1323

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS07200 [old locus tag: SA1270 ]
  • symbol: SA_RS07200
  • description: amino acid permease
  • length: 440
  • theoretical pI: 9.55367
  • theoretical MW: 47261.2
  • GRAVY: 0.935682

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 211.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 170.7)
    and 10 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 133.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 114.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 85.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 72)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 62.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (TIGR00907; HMM-score: 58.8)
    histidine-histamine antiporter (TIGR04298; HMM-score: 54.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 49.5)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 38.8)
    early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 8.2)
  • TheSEED: see SA1270
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 202.8)
    and 2 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 154.4)
    AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 44.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012697
    • TAT(Tat/SPI): 0.000621
    • LIPO(Sec/SPII): 0.01798
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAGMALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYFPANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVIKLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNVAGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFGENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGAIIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYPIIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]