SA RS04395

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS04395 [old locus tag: SA0769 ]
• pan locus tag^?: SAUPAN002838000
• symbol: SA_RS04395
• pan gene symbol^?: metN
• synonym:
• product: methionine import ATP-binding protein MetN 2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS04395 [old locus tag: SA0769 ]
• symbol: SA_RS04395
• product: methionine import ATP-binding protein MetN 2
• replicon: chromosome
• strand: +
• coordinates: 875598..876623
• length: 1026
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (875598..876623) NCBI
• BioCyc: G1G21-880 BioCyc
• MicrobesOnline: see SA0769

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  GTGATTGAATTAAAAGAAGTTGTTAAAGAATATCGGACTAAAAATAAAGAAGTCCTTGCT
  GTAGATCACGTTAATTTATCGATTCGAGCAGGATCGATTTATGGCGTCATTGGTTTTTCT
  GGAGCAGGAAAAAGTACTTTGATTCGAATGTTTAATCATTTAGAAGCGCCTACATCAGGT
  GAAGTTATTATAGATGGAGACCATATAGGTCAATTGTCCAAAAATGGATTAAGAGCAAAA
  AGACAAAAAGTAAATATGATCTTCCAACATTTTAATTTGTTATGGTCAAGGACTGTGTTA
  AAAAATATTATGTTTCCGCTTGAAATTGCAGGTGTCCCTAGAAGGAGAGCTAAGCAAAAA
  GCATTAGAACTTGTCGAACTCGTCGGTTTAAAAGGTAGAGAAAAGGCTTATCCATCAGAG
  TTATCAGGTGGACAAAAGCAACGTGTTGGGATTGCACGAGCGTTAGCTAATGATCCAACG
  GTCTTGCTTTGTGATGAGGCAACAAGTGCACTTGATCCGCAAACAACAGATGAAATTTTA
  GATCTACTACTAAAAATTAGAGAACAACAAAATTTAACAATTGTACTAATTACGCATGAA
  ATGCATGTCATTCGTCGTATTTGTGATGAAGTTGCAGTTATGGAAAGTGGTAAAGTGATA
  GAACATGGACCGGTGACACAGGTTTTTGAAAATCCGCAACACACTGTGACAAAACGATTT
  GTGAAAGAAGATTTAAATGATGATTTCGAAACATCTTTAACAGAATTAGAGCCATTAGAA
  AAAGATGCTTATATCGTTAGATTAGTTTTCGCTGGTTCAACAACAACCGAGCCTATTGTA
  TCGAGTCTATCAACTGCCTATGATATTAAAATTAATATTTTAGAAGCAAATATTAAAAAT
  ACAAAAAATGGAACAGTCGGCTTTTTAGTTCTGCATATTCCATATATTTCAAGTGTAGAT
  TTCGGAAAATTCGAAAAAGAGTTAATTGAGCGACAAGTTAAAATGGAGGTGTTAAGACAT
  GGGTAA
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⊟Protein

⊟General

• locus tag: SA_RS04395 [old locus tag: SA0769 ]
• symbol: SA_RS04395
• description: methionine import ATP-binding protein MetN 2
• length: 341
• theoretical pI: 7.36853
• theoretical MW: 38259
• GRAVY: -0.141642

⊟Function

• reaction:
  EC 3.6.3.-?  ExPASy
• TIGRFAM:
  D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 356.4)
  and 72 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 234.4)
  ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 209.4)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 206.5)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 196.9)
  Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 192.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 191.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 190)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 186.4)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 184.4)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 183.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 173.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 169.5)
  Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 164.7)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 164.2)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 164.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 159.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 159.6)
  ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 157)
  Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 143)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 141.6)
  Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 140)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 140)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 136.2)
  Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 135.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 135.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 134.1)
  Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 134.1)
  Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 132)
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 130.4)
  Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 130.4)
  2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 129.5)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 129.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 129.3)
  gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 128.9)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 128.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 128.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 125.8)
  ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 124)
  thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 119.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 115.1)
  Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 115.1)
  lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 114.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 112.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 108.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 108.3)
  Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 108.3)
  Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 108.3)
  Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 107.4)
  Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 107.4)
  proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 105.8)
  thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 99.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 97.6)
  phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 96.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 94.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 91.3)
  Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 89.5)
  Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 87.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 80.6)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 80.6)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 78)
  Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 76.9)
  ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 70)
  Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 68.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 66.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 66.4)
  Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 66.4)
  Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 44.8)
  Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 43.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 41)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 37.4)
  Metabolism Transport and binding proteins Amino acids, peptides and amines oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain (TIGR01727; HMM-score: 11.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 11.3)
• TheSEED: see SA0769
• PFAM:
  P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 113.1)
  and 10 more
  ACT (CL0070) NIL; NIL domain (PF09383; HMM-score: 50.5)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 27.3)
  AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 26.5)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 21.8)
  SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 21.5)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16)
  Zeta_toxin; Zeta toxin (PF06414; HMM-score: 15.3)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.9)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 13.2)
  AAA_24; AAA domain (PF13479; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010182
  • TAT(Tat/SPI): 0.000889
  • LIPO(Sec/SPII): 0.001392
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446493353 NCBI
• RefSeq: WP_000571207 NCBI
• UniProt: see SA0769

⊟Protein sequence

• MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGKSTLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVNMIFQHFNLLWSRTVLKNIMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPTVLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGKVIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTTEPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVKMEVLRHG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: S-box, CodY see SA0769

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications