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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04125 [old locus tag: SA0724 ]
  • pan locus tag?: SAUPAN002697000
  • symbol: SA_RS04125
  • pan gene symbol?:
  • synonym:
  • product: epimerase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04125 [old locus tag: SA0724 ]
  • symbol: SA_RS04125
  • product: epimerase
  • replicon: chromosome
  • strand: -
  • coordinates: 828460..829362
  • length: 903
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (828460..829362) NCBI
  • BioCyc: SA_RS04125 BioCyc
  • MicrobesOnline: see SA0724

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACAATACTTAATTACTGGTGGGACTGGTATGGTTGGATCTCAATTAGTTAATGAA
    ATTAAAAAATCAGATTCACATATCACGATATTAACGCGACACGACCAAATTTCAAATGAT
    AAGAAAATTTCATATGTCAACTGGGCTAAATCTGGGTGGGAACACAAAGTTCCTCAAAAT
    ATCGATGTGGTCATCAACTTAGCAGGTGCTACACTGAATAAACGATGGACACCGGAATAT
    AAACAAACGCTAATGCTAAGTAGAATTCAATCTACGCAAGCTTTATATGAATTGTTTAAA
    TCACGTAATAAAGCACCTAAAGTTTTATTTAATGCTAGCGCCACTGGTTACTATCCTCCT
    GATTTATTTATGAGTTACACAGAAGTTTATAAAACTTTACCATTTGATTTCTTATCAGAT
    ATTGTGTATCAATGGGAACGTTTCGCACAACAATTTGAACAATTAGGTACGCGAGTTGTC
    ATCGGTCGTTTCGGTATGATTTTATCGAATGAAGGCGGTGCGTTACAAACAATGAAACTA
    CCATACAAATATTACATTGGTGGTAAATTAGGTTCTGGTCAACAATGGTATTCATGGATT
    CATATCAATGATTTAATTCAAGCTATTTTATTTTTAATAAATAACGAGTCAGCTAGTGGT
    CCGTTTAATTTAACTGCACCTATACCTGAGCGTCAAAATTTATTTGGCTACACTTTAGCA
    AGAGCTATGCATAAGCCCCATGAAACTTGGGCACCAAGTCTTGCAATGCGTCTCATACTT
    GGTCAAATGTCAACAGTAGTATTGGATACTCAAAAAGTATTACCTAATAAAATTCAAGCA
    TTGGGATTCCAATTTAAATATAGTAATTTAAAAATGGCACTTGAAGATTTAATCAAAGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS04125 [old locus tag: SA0724 ]
  • symbol: SA_RS04125
  • description: epimerase
  • length: 300
  • theoretical pI: 9.88206
  • theoretical MW: 34272.4
  • GRAVY: -0.229

Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 363.1)
    and 5 more
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 31)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 20.3)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.6)
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.5)
    thioester reductase domain (TIGR01746; HMM-score: 14.5)
  • TheSEED: see SA0724
  • PFAM:
    NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 68.4)
    no clan defined DUF1731; Domain of unknown function (DUF1731) (PF08338; HMM-score: 62)
    and 9 more
    NADP_Rossmann (CL0063) NAD_binding_4; Male sterility protein (PF07993; HMM-score: 25.2)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 20.8)
    KR; KR domain (PF08659; HMM-score: 18.5)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 15.5)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 14.7)
    Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14.4)
    NmrA; NmrA-like family (PF05368; HMM-score: 12.9)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 11.7)
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 11.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029732
    • TAT(Tat/SPI): 0.000589
    • LIPO(Sec/SPII): 0.005143
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKISYVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSRNKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVVIGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPNKIQALGFQFKYSNLKMALEDLIKE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]