From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03425 [old locus tag: SA0596 ]
  • pan locus tag?: SAUPAN002517000
  • symbol: SA_RS03425
  • pan gene symbol?: tagX
  • synonym:
  • product: glycosyl transferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03425 [old locus tag: SA0596 ]
  • symbol: SA_RS03425
  • product: glycosyl transferase
  • replicon: chromosome
  • strand: +
  • coordinates: 689049..690110
  • length: 1062
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (689049..690110) NCBI
  • BioCyc: SA_RS03425 BioCyc
  • MicrobesOnline: see SA0596

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGGCTTACGATAATCATACCTACATGTAATAATGAGGCAACAATTCGACAATTGTTA
    ATATCTATTGAGAGTAAAGAACACTATAGAATCCTTTGTATTGATGGTGGTTCTACTGAT
    CAAACAATTCCTATGATTGAACGGTTACAAAGAGAACTCAAGCATATTTCATTAATACAA
    TTACAAAATGCTTCGATAGCTACGTGTATTAATAAAGGTTTGATGGATATCAAAATGACA
    GATCCACATGATAGTGACGCATTTATGGTCATAAATCCAACATCAATCGTATTGCCAGGT
    AAATTAGATAGGTTAACTGCAGCTTTCAAAAATAATGATAATATTGATATGGTAATAGGG
    CAGCGAGCTTACAATTACCATGGTGAATGGAAATTGAAAAGTGCTGATGAGTTTATTAAA
    GATAATCGAATCGTTACATTAACGGAACAACCAGATTTGTTATCAATGATGTCTTTTGAC
    GGAAAGTTATTCAGTGCTAAATTTGCTGAATTACAGTGTGACGAAACTTTAGCTAACACA
    TACAATCACGCAATACTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCA
    CAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTT
    AATAGATATATCATAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTA
    CCTGAACAAAGGCTATTATATAGTGATATGGTTGATCGTATTTTATTCAATAATTCATTA
    AAATATTATATGAATGAACACCCAGCAGTAACGCACACGACAATTCAACTCGTAAAAGAC
    TATATTATGTCTATGCAGCATTCTGATTATGTATCGCAAAACATGTTTGACATTATAAAT
    ACAGTTGAATTTATTGGTGAGAATTGGGATAGAGAAATATACGAATTGTGGCGACAAACA
    TTAATTCAAGTGGGCATTAATAGGCCGACTTATAAAAAATTCTTGATACAACTTAAAGGG
    AGAAAGTTTGCACATCGAACAAAATCAATGTTAAAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS03425 [old locus tag: SA0596 ]
  • symbol: SA_RS03425
  • description: glycosyl transferase
  • length: 353
  • theoretical pI: 6.74165
  • theoretical MW: 41102.3
  • GRAVY: -0.27932

Function[edit | edit source]

  • reaction:
    EC 2.4.-.-?  ExPASy
  • TIGRFAM:
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 28.7)
    Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 28.6)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 26.9)
    and 1 more
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 16.2)
  • TheSEED: see SA0596
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 43.3)
    and 3 more
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 23.1)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 16.9)
    no clan defined Thg1; tRNAHis guanylyltransferase (PF04446; HMM-score: 15.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016067
    • TAT(Tat/SPI): 0.000134
    • LIPO(Sec/SPII): 0.002928
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIPMIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKLDRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFDGKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDEDFNRYIIEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQLVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKFLIQLKGRKFAHRTKSMLKR

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]