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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02440 [old locus tag: SA0429 ]
  • pan locus tag?: SAUPAN002185000
  • symbol: SA_RS02440
  • pan gene symbol?: gltC
  • synonym:
  • product: LysR family transcriptional regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02440 [old locus tag: SA0429 ]
  • symbol: SA_RS02440
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 489599..490483
  • length: 885
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (489599..490483) NCBI
  • BioCyc: SA_RS02440 BioCyc
  • MicrobesOnline: see SA0429

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAATTAAACAATTAAGATATTTTATCGAGGTCGCTAAAAGAGAACATATCTCCGAA
    ACAGCACTTGAACTAAACATTGCTCAATCAGCGATAAGCCGTCAAATTACATTATTAGAA
    CAAGAACTTAATGTGTCTTTATTTAAAAAGCAAGGACGCAATATTACGCTCACATCTGAA
    GGGAAATTATTATTTAATGAAGCTCTGCGTATTATTGAACATTTAGATAGCACGATTGAA
    CAATTTCAAAGCCATGGATTAACTAAAAATAAAAGTATCTACATTGGCTATGATGAGAGT
    GACGTATCCCACATGTTACTACCACTTATCCAAACGTTTCATTTACAAAATGATACACAT
    GTCATACCGAGCTTATTAGATCATGACACGATTATAAATAGCGTATTAAATGGGAATATA
    GACATTGGTTTCACGGAACTTACACCAGAAATACGTAAACACAAACAATTACATATGCTA
    CCTTTATTCGAAGAGCACTATCATTTATATGCGCCTTCAGACGATCCAATTACGATGGCG
    ACACACCCACCATTAATTCAGTTTGAGCATAGTCATATTTATTGCTTGGCACCATTTGCC
    GAAACTGTAAAAAAACAATTGCGCAAGATTACTAAATCAGATGTATACACAATCAGTAGC
    CAACCACTTGCGCAGTATCTCCTTCGTCAAAAAGAAGGTTACATCATTTCATCACAAAAT
    ATACATTTACCAGAATCTAAATCATGGATAGATATAAAATTAGATCATACTGAATTAAAA
    AGAACGATATGTGCTATTACAAAGGAGCCTTATACAAAGAGTGATATTGGCATATTATTA
    ACACTTATTCAGCAATTAATGACTAAAACGTCTACATTCCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    885

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS02440 [old locus tag: SA0429 ]
  • symbol: SA_RS02440
  • description: LysR family transcriptional regulator
  • length: 294
  • theoretical pI: 6.76422
  • theoretical MW: 33928.8
  • GRAVY: -0.257823

Function[edit | edit source]

  • TIGRFAM:
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 66.4)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 58.8)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 58.8)
    and 5 more
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 50.7)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 40.7)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.4)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.4)
  • TheSEED: see SA0429
  • PFAM:
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 64.2)
    and 4 more
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 32.9)
    no clan defined Ribosomal_S13_N; Ribosomal S13/S15 N-terminal domain (PF08069; HMM-score: 17.2)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 16.3)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by GltC, TF important in Glutamate metabolism: see SA0429

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002278
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000318
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELNVSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDVSHMLLPLIQTFHLQNDTHVIPSLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHMLPLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTISSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDIGILLTLIQQLMTKTSTFH

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY, GltC see SA0429

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]