PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS01460 [old locus tag: SA0248 ]
• pan locus tag^?: SAUPAN001136000
• symbol: SA_RS01460
• pan gene symbol^?: tarS
• synonym:
• product: glycosyl transferase family 2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01460 [old locus tag: SA0248 ]
• symbol: SA_RS01460
• product: glycosyl transferase family 2
• replicon: chromosome
• strand: +
• coordinates: 301718..303439
• length: 1722
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (301718..303439) NCBI
• BioCyc: G1G21-268 BioCyc
• MicrobesOnline: see SA0248

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATGAAATTTTCAGTAATAGTTCCAACATACAATTCAGAAAAGTATATAACAGAATTA
  CTTAATAGCCTTGCGAAACAAGATTTTCCGAAAACTGAATTTGAAGTGGTTGTAGTTGAT
  GACTGTTCAACAGATCAAACGTTACAAATAGTTGAAAAGTATCGCAATAAATTGAACTTG
  AAAGTAAGTCAACTCGAAACAAATTCTGGTGGTCCAGGTAAACCTAGAAATGTGGCGTTA
  AAACAAGCAGAAGGTGAATTTGTATTATTTGTGGACTCCGATGACTATATAAACAAAGAG
  ACTTTAAAGGATGCAGCAGCATTTATTGATGAACATCACTCAGATGTCTTATTGATTAAA
  ATGAAAGGTGTTAATGGTCGTGGTGTACCACAATCTATGTTTAAAGAAACAGCACCTGAA
  GTTACTTTGTTAAATTCAAGAATTATCTATACTTTAAGCCCGACTAAAATCTATAGAACA
  GCATTACTAAAAGATAATGACATTTATTTTCCAGAAGAATTAAAGAGTGCAGAAGATCAA
  TTATTTACAATGAAAGCATATTTGAATGCAAATCGAATCAGTGTGTTAAGTGATAAAGCG
  TATTATTATGCTACAAAGCGTGAAGGTGAACATATGAGTAGTGCGTATGTTTCACCTGAA
  GACTTTTACGAAGTCATGAGATTGATTGCTGTAGAAATATTAAATGCAGATTTAGAAGAA
  GCTCATAAAGATCAAATCTTAGCAGAATTTTTAAATCGTCATTTTAGTTTTTCTCGTACG
  AATGGCTTCTCACTTAAAGTTAAACTTGAAGAGCAACCACAATGGATTAATGCTCTAGGA
  GACTTTATACAAGCAGTTCCAGAACGTGTAGATGCATTGGTGATGAGTAAATTACGACCA
  TTGTTGCACTACGCGAGAGCGAAAGATATAGACAACTATAGAACTGTAGAAGAAAGTTAC
  CGTCAAGGTCAATACTACCGTTTTGATATTGTAGATGGTAAATTAAACATTCAATTCAAT
  GAAGGCGAACCATACTTTGAAGGCATTGATATCGCTAAGCCAAAAGTGAAAATGACAGCA
  TTTAAATTTGATAATCATAAAATTGTTACAGAGCTAACGTTAAATGAATTTATGATTGGC
  GAAGGACATTATGATGTCAGACTTAAATTACATTCACGAAACAAGAAGCACACAATGTAT
  GTACCTTTAAGTGTCAATGCGAATAAACAATATCGTTTTAACATTATGTTAGAAGATATT
  AAAGCGTATTTACCTAAAGAAAAAATTTGGGATGTTTTCTTAGAAGTCCAAATAGGTACG
  GAAGTATTTGAAGTGCGTGTTGGTAATCAACGTAATAAATATGCATATACTGCAGAAACA
  AGTGCATTAATTCATTTAAATAATGATTTTTATAGATTAACACCGTATTTCACAAAAGAC
  TTTAATAACATTTCGTTATACTTTACAGCTATTACATTAACGGATTCAATCTCAATGAAG
  TTAAAAGGTAAAAACAAAATCATTTTAACTGGTCTGGATCGTGGTTATGTATTTGAAGAA
  GGTATGGCTAGTGTCGTGCTAAAAGACGACATGATTATGGGAATGTTAAGCCAAACGTCA
  GAAAACGAAGTGGAAATCTTACTTAGTAAAGATATTAAGAAGCGAGACTTCAAAAATATT
  GTTAAGTTAAACACTGCACATATGACTTACTCGCTAAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS01460 [old locus tag: SA0248 ]
• symbol: SA_RS01460
• description: glycosyl transferase family 2
• length: 573
• theoretical pI: 6.53193
• theoretical MW: 66256.5
• GRAVY: -0.371553

⊟Function

• TIGRFAM:
  putative glycosyltransferase, exosortase G-associated (TIGR03111; HMM-score: 57.6)
  mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 56.9)
  Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 51)
  poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 48.2)
  and 8 more
  chitooligosaccharide synthase NodC (TIGR04242; EC 2.4.1.-; HMM-score: 44.4)
  glycosyltransferase domain (TIGR04440; EC 2.-.-.-; HMM-score: 40.7)
  colanic acid biosynthesis glycosyltransferase WcaA (TIGR04017; EC 2.4.-.-; HMM-score: 36.7)
  hopene-associated glycosyltransferase HpnB (TIGR03469; HMM-score: 28.1)
  colanic acid biosynthesis glycosyltransferase WcaE (TIGR04009; EC 2.4.-.-; HMM-score: 27.7)
  glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 22.2)
  cellulose synthase catalytic subunit (UDP-forming) (TIGR03030; EC 2.4.1.12; HMM-score: 21.7)
  hopanoid biosynthesis associated glycosyl transferase protein HpnI (TIGR03472; HMM-score: 10.7)
• TheSEED: see SA0248
• PFAM:
  GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 112.1)
  and 4 more
  Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 57.8)
  Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 48.4)
  Glyco_tranf_2_4; Glycosyl transferase family 2 (PF13704; HMM-score: 24.8)
  Glyco_transf_49; Glycosyl-transferase for dystroglycan (PF13896; HMM-score: 14.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007451
  • TAT(Tat/SPI): 0.000169
  • LIPO(Sec/SPII): 0.001186
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446898095 NCBI
• RefSeq: WP_000975351 NCBI
• UniProt: see SA0248

⊟Protein sequence

• MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHSRNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQRNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKIILTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTAHMTYSLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications