PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00300 [old locus tag: SA0029 ]
• pan locus tag^?: SAUPAN000059000
• symbol: SA_RS00300
• pan gene symbol^?: pre
• synonym:
• product: Plasmid recombination enzyme type 2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00300 [old locus tag: SA0029 ]
• symbol: SA_RS00300
• product: Plasmid recombination enzyme type 2
• replicon: chromosome
• strand: -
• coordinates: 38428..39690
• length: 1263
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (38428..39690) NCBI
• BioCyc: G1G21-33 BioCyc
• MicrobesOnline: see SA0029

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAGTTATGCAGTTTGTAGAATGCAAAAAGTGAAATCAGCTGGACTAAAAGGCATGCAA
  TTTCATAATCAAAGAGAGCGAAAAAGTAGAACGAATGATGATATTGACCATGAGCGAACA
  CGTGAAAATTATGATTTGAAAAATGATAAAAATATTGATTACAACGAACGTGTCAAAGAA
  ATTATTGAATCACAAAAAACAGGTACAAGAAAAACGAGGAAAGATGCTGTTCTTGTAAAT
  GAGTTGCTAGTAACATCTGACCGAGATTTTTTTGAGCAACTGGATCCAGGAGAACAAAAA
  CGATTTTTTGAGGAAAGTTATAAATTATTTTCCGAACGATATGGCAAGCAAAATATTGCT
  TATGCAACAGTTCATAATGATGAGCAAACCCCTCACATGCATTTAGGTGTTGTGCCTATG
  CGTGATGGAAAACTGCAAGGAAAAAATGTGTTTAATCGTCAAGAACTGTTATGGCTACAA
  GATAAATTCCCCGAGCATATGAAAAAACAGGGTTTTGAGTTGAAGCGTGGTGAACGTGGC
  TCTGACCGTAAACATATTGAGACAGCTAAATTTAAAAAACAAACTTTGGAAAAAGAGATT
  GATTTTCTAGAAAAAAATTTAGCAGTTAAAAAAGATGAATGGACTGCTTATAGCGATAAA
  GTTAAATCAGATTTAGAAGTACCAGCGAAACGACACATGAAAAGTGTTGAAGTGCCAACG
  GGTGAAAAGTCTATGTTTGGTTTGGGAAAAGAAATAATGAAAACAGAAAAGAAACCAACC
  AAAAATGTTGTTATATCGGAGCGTGATTATAAAAACTTAGTGACTGCTGCGAGAGATAAC
  GATAGGTTAAAACAGCATGTTAGAAATCTCATGAGTACTGATATGGCGAGAGAATATAAA
  AAATTAAGTAAAGAACATGGGCAAGTTAAAGAAAAATATAGTGGTCTTGTAGAGCGATTT
  AATGAAAATGTAAATGATTATAATGAGTTGCTTGAAGAAAACAAGTCTTTAAAGTCTAAA
  ATAAGCGATTTAAAGCGTGATGTGAGTTTAATCTATGAAAGCACTAAGGAATTCCTTAAG
  GAACGTACAGACGGCTTAAAAGCCTTTAAAAACGTTTTTAAGGGGTTTGTAGACAAGGTA
  AAGGATAAAACAGCACAATTCCAAGAAAAACACGATTTAGAACCTAAAAAGAACGAATTT
  GAACTAACTCATAACCGAGAGGTAAAAAAAGAACGAAGTCGAGATCAGGGAATGAGTTTA
  TAA
  60
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  1260
  1263
  

⊟Protein

⊟General

• locus tag: SA_RS00300 [old locus tag: SA0029 ]
• symbol: SA_RS00300
• description: Plasmid recombination enzyme type 2
• length: 420
• theoretical pI: 9.97102
• theoretical MW: 49660
• GRAVY: -1.17738

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 5.8)
• TheSEED: see SA0029
• PFAM:
  Rep (CL0169) Mob_Pre; Plasmid recombination enzyme (PF01076; HMM-score: 246.5)
  and 4 more
  no clan defined IncA; IncA protein (PF04156; HMM-score: 16.9)
  Sec34; Sec34-like family (PF04136; HMM-score: 10.8)
  HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 8.7)
  FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 6.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008393
  • TAT(Tat/SPI): 0.002271
  • LIPO(Sec/SPII): 0.004586
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446041550 NCBI
• RefSeq: WP_000119405 NCBI
• UniProt: see SA0029

⊟Protein sequence

• MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications