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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS14765 [old locus tag: SAUSA300_pUSA030006 ]
- pan locus tag?:
- symbol: SAUSA300_RS14765
- pan gene symbol?: —
- synonym:
- product: replication and maintenance protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS14765 [old locus tag: SAUSA300_pUSA030006 ]
- symbol: SAUSA300_RS14765
- product: replication and maintenance protein
- replicon: pUSA03
- strand: +
- coordinates: 6681..7157
- length: 477
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007792 (6681..7157) NCBI
- BioCyc: SAUSA300_RS14765 BioCyc
- MicrobesOnline: see SAUSA300_pUSA030006
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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421ATGAAAGAAAGATATGGAACAGTCTATAAAGGCTCTCAGAGGCTCATAGACGAAGAAAGT
GGAGAAGTCATAGAGGTAGACAAGTTATACCGTAAACAAACGTCTGGTAACTTCGTAAAG
GCATATATAGTGCAATTAATAAGTATGTTAGATATGATTGGCGGAAAAAAACTTAAAATC
GTTAACTATATCCTAGATAATGTCCACTTAAGTAACAATACAATGATAGCTACAACAAGA
GAAATAGCAAAAGCTACAGGAACAAGTCTACAAACAGTAATAACAACACTTAAAATCTTA
GAAGAAGGAAATATTATAAAAAGAAAAACTGGAGTATTAATGTTAAACCCTGAACTACTA
ATGAGAGGCGACGACCAAAAACAAAAATACCTCTTACTCGAATTTGGGAACTTTGAGCAA
GAGGCAAATGAAAAACAAGAAAATGCATTATCTGATTATTATTCTTTCAAGGACTAG60
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477
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS14765 [old locus tag: SAUSA300_pUSA030006 ]
- symbol: SAUSA300_RS14765
- description: replication and maintenance protein
- length: 158
- theoretical pI: 7.49276
- theoretical MW: 18078.7
- GRAVY: -0.411392
⊟Function[edit | edit source]
- TIGRFAM: Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.9)Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly SUF system regulator (TIGR02944; HMM-score: 13.8)Regulatory functions DNA interactions FeS assembly SUF system regulator (TIGR02944; HMM-score: 13.8)
- TheSEED: see SAUSA300_pUSA030006
- PFAM: HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 233.3)and 12 moreHTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 25.1)HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 24.6)FUR; Ferric uptake regulator family (PF01475; HMM-score: 17.1)HTH_29; Winged helix-turn helix (PF13551; HMM-score: 16.4)MarR; MarR family (PF01047; HMM-score: 14.3)HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14)HTH_11; HTH domain (PF08279; HMM-score: 13.6)Rrf2; Transcriptional regulator (PF02082; HMM-score: 13.5)GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 13.4)FaeA; FaeA-like protein (PF04703; HMM-score: 12.8)HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 12.6)HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002149
- TAT(Tat/SPI): 0.000167
- LIPO(Sec/SPII): 0.000509
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446587381 NCBI
- RefSeq: WP_000664727 NCBI
- UniProt: see SAUSA300_pUSA030006
⊟Protein sequence[edit | edit source]
- MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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