PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI date : _

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2605 [new locus tag: SAUSA300_RS14485 ]
• pan locus tag^?: SAUPAN006427000
• symbol: hisIE
• pan gene symbol^?: hisI
• synonym:
• product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2605 [new locus tag: SAUSA300_RS14485 ]
• symbol: hisIE
• product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein
• replicon: chromosome
• strand: -
• coordinates: 2833608..2834240
• length: 633
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914511 NCBI
• RefSeq: YP_495239 NCBI

⊟Phenotype

• Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
  601
  
  ATGACCAAATATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAAT
  CAAACAAAACAAGTATTGATGTTGGGTTATATGAACCAAGAAGCTTTTGATAAAACGATA
  GAAGATGGTGTGGTATGTTTCTATTCGCGGTCGAAACAACGGCTATGGACAAAAGGTGAA
  ACATCTGGTCATACGCAACGTGTTAAAGATATTCATGTAGATTGCGACAATGACACTATT
  TTAATTGATGTCATACCAAATGGACCAACATGTCATACAGGCAGTCAAAGTTGTTTCAAC
  ACAGAAGTTCCATTTTCAGTGCAAACATTAGCGCAGACAGTTCAAGATAGTGCCCAATCC
  AATAATGAAAAGTCATATACAAAATATTTATTAACAGAAGGTATAGAAAAGATTACAAAA
  AAATACGGTGAAGAAGCTTTTGAAGTCGTAATTGAAGCAATTAAAGGTGACAAAAAAGCA
  TTTGTAAGTGAAGTAGCAGATGAACTTTATCATTTATTTGTCTTGATGCATGCGCTTGGC
  GTCGATTTTTCAGAAATTGAGGCGGAATTAGCGCGTAGACATCATAAGCGCAATAACTTT
  AAAGGTGAACGACAAAATATCGAACAGTGGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  633
  

⊟Protein

⊟General

• locus tag: SAUSA300_2605 [new locus tag: SAUSA300_RS14485 ]
• symbol: HisIE
• description: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein
• length: 210
• theoretical pI: 5.899
• theoretical MW: 23986.9
• GRAVY: -0.528571

⊟Function

• reaction:
  EC 3.5.4.19?  ExPASy
  Phosphoribosyl-AMP cyclohydrolase 1-(5-phospho-beta-D-ribosyl)-AMP + H₂O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
  EC 3.6.1.31?  ExPASy
  Phosphoribosyl-ATP diphosphatase 1-(5-phospho-beta-D-ribosyl)-ATP + H₂O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family phosphoribosyl-ATP diphosphatase (TIGR03188; EC 3.6.1.31; HMM-score: 90.8)
• TheSEED  :
  • Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  • Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  and 3 more
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
• PFAM:
  no clan defined PRA-CH; Phosphoribosyl-AMP cyclohydrolase (PF01502; HMM-score: 116.5)
  and 5 more
  MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 51.3)
  MazG-like; MazG-like family (PF12643; HMM-score: 18.7)
  MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 15.1)
  Beta_propeller (CL0186) BBS2_Mid; Ciliary BBSome complex subunit 2, middle region (PF14783; HMM-score: 13.9)
  no clan defined MnmE_helical; MnmE helical domain (PF12631; HMM-score: 12.3)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• interaction partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004252
  • TAT(Tat/SPI): 0.000223
  • LIPO(Sec/SPII): 0.000465
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161165 NCBI
• UniProt: A0A0H2XGN5 UniProt
• protein Genbank : _
• RefSeq: YP_495239 NCBI

⊟Protein sequence

• MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGVVCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTEVPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKAFVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW

⊟Peptides

• experimentally validated:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

⊟Regulation

• sigma factors : _
• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications