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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2602 [new locus tag: SAUSA300_RS14470 ]
  • pan locus tag?: SAUPAN006414000
  • symbol: icaC
  • pan gene symbol?: icaC
  • synonym:
  • product: intercellular adhesion protein C

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2602 [new locus tag: SAUSA300_RS14470 ]
  • symbol: icaC
  • product: intercellular adhesion protein C
  • replicon: chromosome
  • strand: +
  • coordinates: 2828974..2830026
  • length: 1053
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAGATTAGACTTGAACTCGTATATTTACGTGCTATTATATGTGCAATTATTATT
    ATCACACATTTACTTACACAAATTACTTTAAAACATGAAAATATGGAGGGTGGATCCTTA
    GTGTTACAATTTTACATTCGTAATATTGTGATTTTTGGTACACCTTGCTTTATTATCTTG
    TCACAGTTACTGACAACCTTGAATTACCAAAAAGTCACCTATAGATACTTAACTACACGC
    GTAAAATATATACTTATTCCTTACATATTAATGGGATTGTTTTACAGTTATAGTGAATCA
    TTATTAACAGATTCAAGTTTCAATAAACAATTCATTGAAAATGTCCTATTAGGTCAATGG
    TATGGCTATTTTATCGTTGTTATCATGCAATTCTTTATTTTGAGTTATATCATTTTTAAA
    ATTAACTATAACCTATTCAACAGTAAAATATTATTATTGTTATCTTTTATTTTACAGCAA
    TCATTTTTATATTACTTTACGAACAACACAGCGTTTCACGATACCGTGCTACACTATTAT
    CCATTAAGTGAAAATACTATAATATTCGGATGGATTTTTTATTTCTTCTTAGGTGCATAT
    ATGGGTTATAACTACGAACGTGTATTAAATTTCTTAGAACGTTATTTAGTTATTATGATT
    GTATTAGCTGTAGCTACTTATTTTGTGTTTATTGCGTTAGCAAATGGAGACTATTGGAAC
    GTTACCAGCTTTTCATATTCATTAACACCATATAATAGTATTATGTTTATTGTTATCTTG
    GGTATTTGCACGCATTTTAAAACAATGTTATTTAATACGATTCAAATGATTAGTGCTTTC
    TCATTCTTTATTTATTTATTACATCCAATCATTCTAGACTCATTGTTTGCATATACAAAT
    ATATTTGAGGATAATACAATGGTCTTTCTAGCGATATCACTACTATTCATTTTAGGATTA
    TGTATAGGTGTCGGCATGATATTGCGTGAATTCTATATCTTTAGGTTTATTATTGGAAAA
    CAACCATATAAATTGAACATTAATGCTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2602 [new locus tag: SAUSA300_RS14470 ]
  • symbol: IcaC
  • description: intercellular adhesion protein C
  • length: 350
  • theoretical pI: 8.82602
  • theoretical MW: 41344
  • GRAVY: 0.806571

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Polysaccharide intercellular adhesin (PIA) biosynthesis protein IcaC
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Polysaccharide intercellular adhesin (PIA) biosynthesis protein IcaC
  • PFAM:
    Acyl_transf_3 (CL0316) Acyl_transf_3; Acyltransferase family (PF01757; HMM-score: 80)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.078332
    • TAT(Tat/SPI): 0.000265
    • LIPO(Sec/SPII): 0.011994
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQFYIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLLTDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQSFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLNINAY

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: CodY (repression) regulon, IcaR* (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    IcaR*(TF)important in Intercellular adhesion; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Ursula Fluckiger, Martina Ulrich, Andrea Steinhuber, Gerd Döring, Dietrich Mack, Regine Landmann, Christiane Goerke, Christiane Wolz
Biofilm formation, icaADBC transcription, and polysaccharide intercellular adhesin synthesis by staphylococci in a device-related infection model.
Infect Immun: 2005, 73(3);1811-9
[PubMed:15731082] [WorldCat.org] [DOI] (P p)
C Heilmann, O Schweitzer, C Gerke, N Vanittanakom, D Mack, F Götz
Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis.
Mol Microbiol: 1996, 20(5);1083-91
[PubMed:8809760] [WorldCat.org] [DOI] (P p)