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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0666 [new locus tag: SAUSA300_RS03575 ]
  • pan locus tag?: SAUPAN002564000
  • symbol: SAUSA300_0666
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0666 [new locus tag: SAUSA300_RS03575 ]
  • symbol: SAUSA300_0666
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 739923..740489
  • length: 567
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAACGAATCGCAGTTTATTGTGGTGCAAGCAAAGGTCATGACCCTTCATATGTACAA
    AAAGCATACGATTTAGGTAAATATTTTGCTGAACAAGGTTATGAATTAGTATTTGGTGCA
    GGGTCAATTGGCATTATGGGTGCCATTCAAGATGGTGTATTAGATCATGGCGGCAAAGCT
    ATTGGCGTCATGCCTAAAATGTTAGATGAACATGAAATCACAAGCCAACGTTTAACTGAA
    TTAATTTTAGTTGATTCTATGCATGAACGTAAAAATAAAATGGCAGAACTTGCAGATGCA
    TTTGTCATGGCTCCTGGTGGCGCAGGTTCACTAGAAGAATTTTTCGAAATGTATAGTTGG
    GCTCAAATAGGTATCCATGAAAAGCCTATTGCCATTTATAATATTAATGGCTTTTTCAAT
    CCACTACAAACAATGATTGACCATATGATTGAGGAAGGTTTTATTGATCCTAAGTATCGT
    GCTCTTGCACCGTTATGCGACACTAAGGAATCATTAATTGAATCAATATTAAATTTCAAA
    CCTCTGGGTACTCGTTCATACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0666 [new locus tag: SAUSA300_RS03575 ]
  • symbol: SAUSA300_0666
  • description: hypothetical protein
  • length: 188
  • theoretical pI: 4.89275
  • theoretical MW: 20888.9
  • GRAVY: -0.114894

Function[edit | edit source]

  • reaction:
    EC 3.2.2.n1?  ExPASy
    Cytokinin riboside 5'-monophosphate phosphoribohydrolase N6-(Delta2-isopentenyl)-adenosine 5'-phosphate + H2O = N6-(dimethylallyl)adenine + D-ribose 5'-phosphate Trans-zeatin riboside phosphate + H2O = trans-zeatin + D-ribose 5'-phosphate
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00730 family protein (TIGR00730; HMM-score: 197.5)
    and 3 more
    Hypothetical proteins Conserved TIGR00725 family protein (TIGR00725; HMM-score: 37.6)
    Cellular processes Cellular processes DNA transformation DNA protecting protein DprA (TIGR00732; HMM-score: 13)
    gliding motility-associated lipoprotein GldK (TIGR03525; HMM-score: 12.6)
  • TheSEED  :
    • Lysine decarboxylase family
  • PFAM:
    DprA (CL0349) Lysine_decarbox; Possible lysine decarboxylase (PF03641; HMM-score: 151.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017898
    • TAT(Tat/SPI): 0.000237
    • LIPO(Sec/SPII): 0.000832
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIGIMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFVMAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYRALAPLCDTKESLIESILNFKPLGTRSYD

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]