COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SAP016 [new locus tag: SA_RS00070 ]
• pan locus tag^?:
• symbol: arsR
• pan gene symbol^?: —
• synonym:
• product: arsenical resistance operon repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAP016 [new locus tag: SA_RS00070 ]
• symbol: arsR
• product: arsenical resistance operon repressor
• replicon: pN315
• strand: +
• coordinates: 14107..14421
• length: 315
• essential: unknown

⊟Accession numbers

• Gene ID: 1122761 NCBI
• RefSeq: NP_395552 NCBI
• BioCyc: see SA_RS00070
• MicrobesOnline: 143486 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGTCTTATAAAGAACTATCAACAATATTAAAAATTTTATCAGATCCAAGTAGGTTAGAA
  ATATTAGATTTACTTTCTTGTGGTGAGCTATGCGCTTGTGACTTATTAGAACACTTTCAA
  TTCTCACAACCTACACTAAGTCATCATATGAAGTCATTAGTAGATAATGAATTAGTTACA
  ACACGAAAAGATGGCAATAAACATTGGTATCAACTTAATCATGCTATTTTAGATGATATT
  ATCCAAAACTTGAACATCATTAATACATCTAATCAAAGATGTGTATGTAAAAATGTGAAA
  TCAGGTGACTGTTGA
  60
  120
  180
  240
  300
  315
  

⊟Protein

⊟General

• locus tag: SAP016 [new locus tag: SA_RS00070 ]
• symbol: ArsR
• description: arsenical resistance operon repressor
• length: 104
• theoretical pI: 6.3292
• theoretical MW: 11873.6
• GRAVY: -0.246154

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 14.9)
  Signal transduction Regulatory functions DNA interactions copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 14.9)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 13.3)
  and 1 more
  Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 10.1)
• TheSEED  :
  • Arsenical resistance operon repressor
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Arsenic resistance  Arsenical resistance operon repressor
• PFAM:
  HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 58)
  and 8 more
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 45)
  MarR_2; MarR family (PF12802; HMM-score: 18.9)
  F-box (CL0271) F-box; F-box domain (PF00646; HMM-score: 16.1)
  HTH (CL0123) TFIIE_alpha; TFIIE alpha subunit (PF02002; HMM-score: 15.7)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 15.5)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.1)
  RP-C; Replication protein C N-terminal domain (PF03428; HMM-score: 13.7)
  MarR; MarR family (PF01047; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors: Arsenate; Arsenite
• genes regulated by ArsR, TF important in Arsenic resistanceRegPrecise

  repression

  arsR > arsB > arsC

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024844
  • TAT(Tat/SPI): 0.002066
  • LIPO(Sec/SPII): 0.084443
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 16119216 NCBI
• RefSeq: NP_395552 NCBI
• UniProt: A0A0H3JNP1 UniProt

⊟Protein sequence

• MSYKELSTILKILSDPSRLEILDLLSCGELCACDLLEHFQFSQPTLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVKSGDC

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: arsR > arsB > arsC

⊟Regulation

• regulator: ArsR (repression) regulon

  ArsR

  (TF)

  important in Arsenic resistance; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications