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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02969
  • pan locus tag?: SAUPAN006364000
  • symbol: SAOUHSC_02969
  • pan gene symbol?: arcA
  • synonym:
  • product: arginine deiminase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02969
  • symbol: SAOUHSC_02969
  • product: arginine deiminase
  • replicon: chromosome
  • strand: -
  • coordinates: 2730276..2731511
  • length: 1236
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAGATGGTCCAATTAAAGTAAATAGCGAAATTGGAGCTTTAAAAACTGTGTTACTT
    AAGCGTCCTGGAAAAGAATTAGAAAATTTAGTACCTGATTATTTAGATGGATTACTATTT
    GATGATATTCCATATTTAGAAGTAGCTCAAAAAGAGCATGACCATTTTGCGCAGGTGCTA
    AGAGAAGAGGGTGTTGAAGTACTTTACCTTGAGAAGTTAGCAGCTGAAAGTATTGAAAAT
    CCTCAAGTAAGAAGTGAATTTATTGATGATGTATTAGCAGAGTCTAAAAAAACAATATTA
    GGTCATGAAGAAGAAATTAAGGCATTATTTGCGACACTTTCTAATCAAGAACTTGTAGAT
    AAAATAATGTCAGGGGTACGTAAGGAAGAAATTAATCCGAAATGTACACATCTAGTAGAG
    TATATGGATGATAAGTATCCATTCTATTTAGATCCAATGCCAAACCTTTATTTTACTAGA
    GATCCACAAGCCTCAATAGGACACGGTATAACAATCAATCGGATGTTCTGGAGAGCACGA
    CGACGAGAATCAATATTTATTCAATATATTGTAAAGCATCATCCTAGATTTAAAGATGCG
    AATATTCCAATCTGGTTAGATCGAGATTGCCCATTCAATATTGAAGGCGGCGATGAACTT
    GTTTTATCTAAAGATGTCTTGGCTATAGGCGTTTCAGAACGTACATCTGCACAAGCTATT
    GAAAAGTTAGCGCGACGTATTTTTGAAAATCCGCAGGCGACGTTTAAAAAAGTAGTAGCA
    ATTGAAATTCCAACTAGTCGAACTTTTATGCACTTAGATACAGTATTTACAATGATAGAT
    TATGACAAATTTACAATGCATTCAGCCATTTTAAAGGCAGAAGGCAATATGAATATATTT
    ATTATTGAATATGATGACGTAAATAAAGATATTGCCATCAAACAATCTAGTCATTTAAAA
    GATACTTTAGAAGACGTACTAGGTATAGATGATATCCAATTCATTCCAACAGGAAATGGT
    GATGTCATTGATGGTGCTAGAGAGCAATGGAATGATGGCTCAAATACATTATGTATAAGA
    CCAGGCGTTGTAGTGACTTACGATAGAAACTATGTATCGAATGATTTATTGAGACAAAAA
    GGCATTAAAGTCATTGAAATATCTGGTAGCGAGTTGGTACGTGGACGTGGGGGCCCTAGA
    TGTATGAGTCAACCATTATTCAGAGAGGACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1236

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02969
  • symbol: SAOUHSC_02969
  • description: arginine deiminase
  • length: 411
  • theoretical pI: 4.87271
  • theoretical MW: 46914.3
  • GRAVY: -0.310706

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.3.6?  ExPASy
    Arginine deiminase L-arginine + H2O = L-citrulline + NH3
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines arginine deiminase (TIGR01078; EC 3.5.3.6; HMM-score: 544.2)
  • TheSEED  :
    • Arginine deiminase (EC 3.5.3.6)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine deiminase (EC 3.5.3.6)
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine deiminase (EC 3.5.3.6)
  • PFAM:
    GME (CL0197) Amidinotransf; Amidinotransferase (PF02274; HMM-score: 400.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001529
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.000227
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIPYLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGHEEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTRDPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGDELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVFTMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQFIPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSELVRGRGGPRCMSQPLFREDI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SAOUHSC_01722(alaS)alanyl-tRNA synthetase  [3] (data from MRSA252)
    SAOUHSC_00799(eno)phosphopyruvate hydratase  [3] (data from MRSA252)
    SAOUHSC_00520(rplJ)50S ribosomal protein L10  [3] (data from MRSA252)
    SAOUHSC_02507(rplV)50S ribosomal protein L22  [3] (data from MRSA252)
    SAOUHSC_00769(secA)preprotein translocase subunit SecA  [3] (data from MRSA252)
    SAOUHSC_0047450S ribosomal protein L25/general stress protein Ctc  [3] (data from MRSA252)
    SAOUHSC_0052830S ribosomal protein S7  [3] (data from MRSA252)
    SAOUHSC_00690hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [3] (data from MRSA252)
    SAOUHSC_01041pyruvate dehydrogenase complex, E1 component subunit beta  [3] (data from MRSA252)
    SAOUHSC_01042branched-chain alpha-keto acid dehydrogenase subunit E2  [3] (data from MRSA252)
    SAOUHSC_01416dihydrolipoamide succinyltransferase  [3] (data from MRSA252)
    SAOUHSC_01490DNA-binding protein HU  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR* (repression) regulon, CcpA* regulon, Rex* (repression) regulon, ArcR* (activation) regulon
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise 
    Rex*(TF)important in Energy metabolism; RegPrecise 
    ArcR*(TF)important in Arginine degradation; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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