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Revision as of 11:05, 10 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02962
  • pan locus tag?: SAUPAN006356000
  • symbol: SAOUHSC_02962
  • pan gene symbol?: estA
  • synonym:
  • product: tributyrin esterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02962
  • symbol: SAOUHSC_02962
  • product: tributyrin esterase
  • replicon: chromosome
  • strand: -
  • coordinates: 2722091..2722852
  • length: 762
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3921664 NCBI
  • RefSeq: YP_501413 NCBI

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCTTATATTTCATTAAACTATCATTCACCAACAATTGGTATGCATCAAAATTTGACA
    GTCATTTTACCGGAAGATCAAAGCTTCTTTAATAGCGATACAACTGTTAAACCATTAAAA
    ACTTTAATGTTGTTACATGGATTATCAAGTGATGAAACGACATATATGAGATATACAAGC
    ATAGAAAGGTATGCGAATGAACACAAATTAGCTGTGATTATGCCCAATGTGGATCATAGC
    GCATATGCTAACATGGCATATGGTCATAGCTATTATGATTATATTTTGGAAGTGTATGAT
    TATGTTCATCAAATATTTCCACTTTCCAAAAAGCGTGATGACAATTTTATAGCAGGTCAC
    TCTATGGGAGGATATGGCACAATTAAATTTGCATTAACACAAGGGGATAAATTTGCCAAA
    GCTGTACCATTATCTGCTGTGTTTGAAGCGCAAAATTTAATGGATCTAGAGTGGAATGAT
    TTTTCAAAAGAGGCCATAATTGGCAATCTTTCAAGTGTTAAAGGAACTGAACATGATCCG
    TATTACTTGCTAGACAAAGCTGTAGCTGAAGATAAACAAATTCCAAAATTGCTCATTATG
    TGTGGTAAACAAGACTTTTTATATCAAGACAACTTAGATTTTATCGATTATTTATCACGC
    ATAAATGTTCCTTATCAATTTGAAGATGGACCAGGAGATCATGATTATGCATATTGGGAT
    CAAGCGATTAAGCGTGCTATAACATGGATGGTGAATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    762

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02962
  • symbol: SAOUHSC_02962
  • description: tributyrin esterase
  • length: 253
  • theoretical pI: 4.82893
  • theoretical MW: 29095.7
  • GRAVY: -0.334387

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification S-formylglutathione hydrolase (TIGR02821; EC 3.1.2.12; HMM-score: 44.5)
    and 3 more
    Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 19.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 16.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 13.8)
  • TheSEED  :
    • Tributyrin esterase
  • PFAM:
    AB_hydrolase (CL0028) Esterase; Putative esterase (PF00756; HMM-score: 55.8)
    and 7 more
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 28.1)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 26.9)
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 16.3)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 14.1)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 13.8)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 13.6)
    DHFred (CL0387) DHFR_1; Dihydrofolate reductase (PF00186; HMM-score: 11.7)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002008
    • TAT(Tat/SPI): 0.000116
    • LIPO(Sec/SPII): 0.000385
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 88196584 NCBI
  • UniProt: Q2FUY3 UniProt
  • protein Genbank : _
  • RefSeq: YP_501413 NCBI

⊟Protein sequence[edit | edit source]

  • MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLMLLHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYVHQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWNDFSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYLSRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND

⊟Peptides[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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