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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02244
  • pan locus tag?: SAUPAN005193000
  • symbol: SAOUHSC_02244
  • pan gene symbol?: —
  • synonym:
  • product: succinyl-diaminopimelate desuccinylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02244
  • symbol: SAOUHSC_02244
  • product: succinyl-diaminopimelate desuccinylase
  • replicon: chromosome
  • strand: +
  • coordinates: 2078221..2079444
  • length: 1224
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAACTTTTAGTGAAAAAGAAAAAATTCAATTACTAGCAGATATTGTTGAACTACAA
    ACTGAAAATAATAATGAAATAGACGTTTGTAATTATTTAACAGATTTATTCGACAAGTAC
    GATATTAAATCTGAAATTTTGAAAGTTAATGAACACCGCGCCAATATCGTTGCAGAAATC
    GGTAACGGCTCACCTATACTCGCATTGAGTGGTCATATGGATGTTGTTGATGCAGGAAAT
    CAAGATAATTGGTCATATCCCCCTTTTCAACTGACAGAAAAAGATGGCAAATTATATGGC
    CGAGGCACTACAGATATGAAAGGCGGTTTAATGGCTTTGGTCGTATCTCTAATCGAATTA
    AAAGAACAAAATGAATTGCCTCATGGAACGATTAGATTACTGGCTACTGCTGGCGAAGAG
    AAAGAACAAGAAGGTGCCAAATTATTAGCTGATAAAGGCTATTTAGACGATGTCGATGGC
    TTAATTATTGCTGAACCAACTGGATCTGGAATTTATTATGCACATAAGGGGTCTATGTCA
    TGTAAAGTAACTGCAACTGGTAAAGCTGTCCATAGCTCAGTTCCATTTATTGGTGACAAT
    GCAATTGATACACTGCTTGAATTTTATAATCTATTTAAAGAAAAATATTCAGAGCTTAAA
    CAACAAGATACTAAACATGAATTAGATGTTGCGCCTATGTTCAAATCATTGATTGGAAAA
    GAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATGTTCGATTATA
    AATGGCGGCAAACAATTTAACTCTGTACCAGATGAAGCTTCACTTGAATTTAACGTAAGA
    CCAGTTCCTGAGTATGATAACGACTTTATAGAATCGTTTTTCCAAAATATCATTAATGAT
    GTGGATAGCAATAAGCTTTCACTCGATATTCCAAGCAATCACCGACCTGTAACAAGCGAT
    AAAAATAGCAAATTAATTACTACGATTAAAGATGTAGCTTCTAGTTATGTAGAACAAGAC
    GAAATATTTGTTTCAGCGCTTGTAGGCGCAACAGATGCCTCTAGTTTCTTAGGAGATAAT
    AAGGACAATGTTGATTTAGCCATTTTTGGACCAGGTAATCCATTAATGGCACATCAAATC
    GATGAATATATTGAAAAAGATATGTATCTGAAATATATTGATATTTTTAAAGAGGCTTCC
    ATTCAATATTTAAAAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1224

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02244
  • symbol: SAOUHSC_02244
  • description: succinyl-diaminopimelate desuccinylase
  • length: 407
  • theoretical pI: 4.27856
  • theoretical MW: 45097.3
  • GRAVY: -0.329975

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 300.7)
    and 11 more
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 167.9)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 134.5)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 122)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 114.6)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 100.7)
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 95.3)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 78.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 75.6)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 51.8)
    peptidase T-like protein (TIGR01883; HMM-score: 31.3)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 29.2)
  • TheSEED  :
    • Acetylornithine deacetylase (EC 3.5.1.16)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Acetylornithine deacetylase (EC 3.5.1.16)
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 137.5)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 81.4)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 21.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003302
    • TAT(Tat/SPI): 0.000133
    • LIPO(Sec/SPII): 0.000511
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKSEILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRGTTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSELKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEASIQYLKEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [3] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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