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Revision as of 18:57, 10 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02225
  • symbol: SAOUHSC_02225
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2068045..2068365
  • length: 321
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Gene ID: 3919648 NCBI
  • RefSeq: YP_500710 NCBI

Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGCCACATATTTTAAACGTAACAGTTCCAATACCTGAAACACATGTACTTATCACAAAA
    GATGAATATGATGAGCTAATTGGTTATTCATTAGACCCTGTATGGAACATGAGTGACTTA
    AAGAAGAAATTAAAAATTGCATCTGATGAGACTATCAAGGACAGATTACTATTTCATCCT
    AGATTTGAAAAAGAACTAAGAGCGCAAGGAATTGTGCATTACCCAGATGAGAATTTTAAT
    CGCTGGAGATTTAACGCAAGAAAGATGAATAAATTCGTCGATGAGCATTTCAATGAAATA
    TATAAGGAGAGAATAAAATGA
    60
    120
    180
    240
    300
    321

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02225
  • symbol: SAOUHSC_02225
  • description: hypothetical protein
  • length: 106
  • theoretical pI: 7.88622
  • theoretical MW: 12854.7
  • GRAVY: -0.718868

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Phage protein
  • PFAM:
    no clan defined DUF771; Domain of unknown function (DUF771) (PF05595; HMM-score: 104.6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002424
    • TAT(Tat/SPI): 0.000093
    • LIPO(Sec/SPII): 0.00037
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 88195899 NCBI
  • UniProt: Q2FWQ5 UniProt
  • protein Genbank : _
  • RefSeq: YP_500710 NCBI

Protein sequence[edit | edit source]

  • MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKLKIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYKERIK

Peptides[edit | edit source]

  • experimentally validated:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • sigma factors : _
  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]