NCBI date : _
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01987
- pan locus tag?: SAUPAN004790000
- symbol: SAOUHSC_01987
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01987
- symbol: SAOUHSC_01987
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1890834..1891433
- length: 600
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
- Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGAT
ATTATTAGAAAGCAATTTAACAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACA
GATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTGAAAAACATGCTGTTGATTTT
AGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGAT
AAGATTAAAACTAAAGAAAATAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAG
CAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGGAAGCAAGAGAAATTTTCTTA
GTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAA
GAAGCGGCTGCAGGTTTACCTGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA60
120
180
240
300
360
420
480
540
600
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01987
- symbol: SAOUHSC_01987
- description: hypothetical protein
- length: 199
- theoretical pI: 5.14542
- theoretical MW: 22343.3
- GRAVY: -0.331658
⊟Function[edit | edit source]
- TIGRFAM: Central intermediary metabolism Amino sugars glucosamine-6-phosphate deaminase (TIGR00502; EC 3.5.99.6; HMM-score: 33.2)and 3 moreEnergy metabolism Pentose phosphate pathway 6-phosphogluconolactonase (TIGR01198; EC 3.1.1.31; HMM-score: 19.1)type-F conjugative transfer system protein TraW (TIGR02743; HMM-score: 12.7)glutaredoxin-like protein NrdH (TIGR02194; HMM-score: 12.5)
- TheSEED :
- Hypothetical protein SAV1854
- PFAM: ISOCOT_Fold (CL0246) Glucosamine_iso; Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase (PF01182; HMM-score: 17)no clan defined DUF2103; Predicted metal-binding protein (DUF2103) (PF09876; HMM-score: 15.3)and 1 moreGlnB-like (CL0089) DUF3240; Protein of unknown function (DUF3240) (PF11582; HMM-score: 13.1)
⊟Structure, modifications & interactions[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- interaction partners:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002114
- TAT(Tat/SPI): 0.000106
- LIPO(Sec/SPII): 0.000336
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGKTSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA
⊟Peptides[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01986 < SAOUHSC_01987predicted SigA promoter [3] : S790 < SAOUHSC_01986 < SAOUHSC_01987 < S791 < SAOUHSC_01988 < SAOUHSC_01989
⊟Regulation[edit | edit source]
- sigma factors : _
- regulator: SigB* (activation) regulon
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 3.2 3.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e) - ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p)