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Revision as of 00:59, 11 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01384
  • pan locus tag?: SAUPAN003793000
  • symbol: SAOUHSC_01384
  • pan gene symbol?: phoU
  • synonym:
  • product: PhoU family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01384
  • symbol: SAOUHSC_01384
  • product: PhoU family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 1327781..1328422
  • length: 642
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3920793 NCBI
  • RefSeq: YP_499911 NCBI

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAATAATTAGACAACGATATCAGGAGCAACTTGATGATTTAATAAAAGAATTACGT
    CGGTTAGGTGCAAATGTCTATGTGAGTATTGAAAATGGTATAAAATCATTAAGTATTGAC
    GATAGAGGCTTTGCACGACAAACAGTTAAAAACGATAAACATATCAATCAATTAAATTAT
    GATATTAATGAGCGAGTTATCATGTTAATTACAAAGCAACAGCCCATTGCGAGTGATTTG
    CGTATGATGATTTCTTCATTAAAAATCGCCTCCGATTTAGAAAGAATAGGAGATAATGCC
    TCGAGTATTGCCAATATTCGATTGCGTACAAAGATTACAGATGATTATGTGTTAACCCGT
    TTAAAGACAATGGGTAAATTAGCTATGTTAATGTTAAAGGACTTAGATCAAGCATTTAAA
    AAGAAAGATACCGTATTAATAAGAGAAATAATTGAGCGTGATGAAGATATCGATGACTTA
    TATAGTCATATTATTAACGCAACGTATCTTATTGATAACGATCCATTTGTCGCTGCACAA
    GCTCATTTAGCAGCAAGACATTTAGAACGTATTGGTGATCATATTATTAACATCGCTGAA
    AGTGTTTATTTTTATTTAACAGGTACACATTACGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01384
  • symbol: SAOUHSC_01384
  • description: PhoU family transcriptional regulator
  • length: 213
  • theoretical pI: 6.5234
  • theoretical MW: 24619.1
  • GRAVY: -0.284507

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
    Signal transduction Regulatory functions Other phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
  • TheSEED  :
    • Phosphate transport system regulatory protein PhoU
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate transport system regulatory protein PhoU
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate transport system regulatory protein PhoU
  • PFAM:
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 125)
    and 2 more
    no clan defined Bclx_interact; Bcl-x interacting, BH3 domain (PF08945; HMM-score: 10)
    Met_repress (CL0057) SeqA_N; SeqA protein N-terminal domain (PF17206; HMM-score: 8.4)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006684
    • TAT(Tat/SPI): 0.000812
    • LIPO(Sec/SPII): 0.000704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 88195111 NCBI
  • UniProt: Q2FYQ1 UniProt
  • protein Genbank : _
  • RefSeq: YP_499911 NCBI

⊟Protein sequence[edit | edit source]

  • MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGFARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASSIANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDLYSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ

⊟Peptides[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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