NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01249
- pan locus tag?: SAUPAN003573000
- symbol: SAOUHSC_01249
- pan gene symbol?: ribC
- synonym:
- product: riboflavin biosynthesis protein RibF
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3919980 NCBI
- RefSeq: YP_499782 NCBI
- BioCyc: G1I0R-1167 BioCyc
- MicrobesOnline: 1289696 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAAGTCATAGAAGTGACACATCCTATACAATCTAAACAGTATATTACAGAGGATGTT
GCAATGGCATTCGGATTTTTCGATGGCATGCATAAAGGTCATGACAAAGTCTTTGATATA
TTAAACGAAATAGCTGAGGCACGCAGTTTAAAAAAAGCGGTGATGACATTTGATCCGCAT
CCGTCTGTCGTGTTGAATCCTAAAAGAAAACGAACAACGTATTTAACGCCACTTTCAGAT
AAAATCGAAAAAATTAGCCAACATGATATTGATTATTGTATAGTGGTTAATTTTTCATCT
AGGTTTGCTAATGTGAGCGTAGAAGATTTTGTTGAAAATTATATAATTAAAAATAATGTA
AAAGAAGTCATTGCTGGTTTTGATTTTACTTTTGGTAAATTTGGAAAAGGTAATATGACT
GTACTTCAAGAATATGATGCGTTTAATACGACAATTGTGAGTAAACAAGAAATTGAAAAT
GAAAAAATTTCTACAACTTCTATTCGTCAAGATTTAATCAATGGTGAGTTGCAAAAAGCG
AATGATGCTTTAGGCTATATATATTCTATTAAAGGCACTGTAGTGCAAGGTGAAAAAAGG
GGAAGAACTATTGGCTTCCCAACAGCTAACATTCAACCTAGTGATGATTATTTGTTACCT
CGTAAAGGTGTTTATGCTGTTAGTATTGAAATCGGCACTGAAAATAAATTATATCGAGGG
GTAGCTAACATAGGTGTAAAGCCAACATTTCATGATCCTAACAAAGCAGAAGTTGTCATC
GAAGTGAATATCTTTGACTTTGAGGATAATATTTATGGTGAACGAGTGACCGTGAATTGG
CATCATTTCTTACGTCCTGAGATTAAATTTGATGGTATCGACCCATTAGTTAAACAAATG
AACGATGATAAATCGCGTGCTAAATATTTATTAGCAGTTGATTTTGGTGATGAAGTAGCT
TATAATATCTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01249
- symbol: SAOUHSC_01249
- description: riboflavin biosynthesis protein RibF
- length: 323
- theoretical pI: 5.89394
- theoretical MW: 36691.4
- GRAVY: -0.314861
⊟Function[edit | edit source]
- reaction: EC 2.7.7.2? ExPASyFAD synthetase ATP + FMN = diphosphate + FADEC 2.7.1.26? ExPASyRiboflavin kinase ATP + riboflavin = ADP + FMN
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibF (TIGR00083; EC 2.7.1.26,2.7.7.2; HMM-score: 286.3)and 1 morecytidyltransferase-like domain (TIGR00125; HMM-score: 15.7)
- TheSEED :
- FMN adenylyltransferase (EC 2.7.7.2)
- Riboflavin kinase (EC 2.7.1.26)
Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD riboflavin to FAD FMN adenylyltransferase (EC 2.7.7.2)and 4 moreCofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD riboflavin to FAD Riboflavin kinase (EC 2.7.1.26)Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD Riboflavin, FMN and FAD metabolism Extended FMN adenylyltransferase (EC 2.7.7.2) - PFAM: HUP (CL0039) FAD_syn; FAD synthetase (PF06574; HMM-score: 164.4)and 2 moreno clan defined Flavokinase; Riboflavin kinase (PF01687; HMM-score: 124.5)HUP (CL0039) CTP_transf_like; Cytidylyltransferase-like (PF01467; HMM-score: 15.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004761
- TAT(Tat/SPI): 0.000202
- LIPO(Sec/SPII): 0.0003
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEIAEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRFANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIENEKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYLLPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERVTVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: rbfA > SAOUHSC_01248 > SAOUHSC_01249 > rpsOpredicted SigA promoter [4] : S517 > rbfA > S518 > SAOUHSC_01248 > SAOUHSC_01249 > S519 > rpsO > S520 > SAOUHSC_01251predicted SigB promoter [4] : S518 > SAOUHSC_01248 > SAOUHSC_01249 > S519 > rpsO > S520 > SAOUHSC_01251
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 4.0 4.1 4.2 4.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)