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Revision as of 13:18, 10 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00688
  • symbol: SAOUHSC_00688
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 672925..673491
  • length: 567
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Gene ID: 3920992 NCBI
  • RefSeq: YP_499247 NCBI

Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAACGAATCGCAGTTTATTGTGGTGCAAGCAAAGGTCATGACCCTTCATATGTACAA
    AAAGCATACGATTTAGGTAAATATTTTGCTGAACAAGGTTATGAATTAGTATTTGGTGCA
    GGGTCAATTGGCATTATGGGTGCCATTCAAGATGGTGTATTAGATCATGGCGGCAAAGCT
    ATTGGCGTCATGCCTAAAATGTTAGATGAACATGAAATCACAAGCCAACGTTTAACTGAA
    TTAATTTTAGTTGATTCTATGCATGAACGTAAAAATAAAATGGCAGAACTTGCAGATGCA
    TTTGTCATGGCTCCTGGTGGCGCAGGTTCACTAGAAGAATTTTTCGAAATGTATAGTTGG
    GCTCAAATAGGTATCCATGAAAAGCCTATTGCCATTTATAATATTAATGGCTTTTTCAAT
    CCACTACAAACAATGATTGACCATATGATTGAGGAAGGTTTTATTGATCCTAAGTATCGT
    GCTCTTGCACCGTTATGCGACACTAAGGAATCATTAATTGAATCAATATTAAATTTCAAA
    CCTCTGGGTACTCGTTCATACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00688
  • symbol: SAOUHSC_00688
  • description: hypothetical protein
  • length: 188
  • theoretical pI: 4.89275
  • theoretical MW: 20888.9
  • GRAVY: -0.114894

Function[edit | edit source]

  • reaction:
    EC 3.2.2.n1?  ExPASy
    Cytokinin riboside 5'-monophosphate phosphoribohydrolase N6-(Delta2-isopentenyl)-adenosine 5'-phosphate + H2O = N6-(dimethylallyl)adenine + D-ribose 5'-phosphate Trans-zeatin riboside phosphate + H2O = trans-zeatin + D-ribose 5'-phosphate
  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00730 family protein (TIGR00730; HMM-score: 197.5)
    and 3 more
    Hypothetical proteins Conserved TIGR00725 family protein (TIGR00725; HMM-score: 37.6)
    Cellular processes Cellular processes DNA transformation DNA protecting protein DprA (TIGR00732; HMM-score: 13)
    gliding motility-associated lipoprotein GldK (TIGR03525; HMM-score: 12.6)
  • TheSEED  :
    • Lysine decarboxylase family
  • PFAM:
    DprA (CL0349) Lysine_decarbox; Possible lysine decarboxylase (PF03641; HMM-score: 151.5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017898
    • TAT(Tat/SPI): 0.000237
    • LIPO(Sec/SPII): 0.000832
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 88194453 NCBI
  • UniProt: Q2G0B7 UniProt
  • protein Genbank : _
  • RefSeq: YP_499247 NCBI

Protein sequence[edit | edit source]

  • MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIGIMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFVMAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYRALAPLCDTKESLIESILNFKPLGTRSYD

Peptides[edit | edit source]

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • sigma factors : _
  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [4] [3]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]