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Revision as of 12:08, 12 January 2016

NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00319
  • pan locus tag?: SAUPAN001875000
  • symbol: SAOUHSC_00319
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00319
  • symbol: SAOUHSC_00319
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 333952..335013
  • length: 1062
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3919585 NCBI
  • gene Genbank : _

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCCAAATTAGAAATGAATAAAAATACGCCTCTTGAGTTTGGTTTGTATTCCTTAGGT
    GATCATTTATTGAATCCATTGAAAGGTGAAAAAGTTAGTTATGAGCAACGTATTAATGAA
    ATTATTGAAGCAAGTAAATTAGCAGATGAAGCAGGTATTGATGTTTTTGCAGTTGGTGAA
    AGTCATCAGGAGCATTTTACAACACAGGCACATACGGTTGTGTTAGGTGCAATTGCCCAA
    GCGACAAAGCATATTAAAGTTTCAAGTTCTTCAACGATTATTAGTGCAACAGATCCTGTA
    AGAGTATTTGAAGACTTCGCGACATTAGATTTGATTTCTCATGGTAGAGCCGAAATTGTA
    GCTGGCAGAGCATCAAGAACAGGTATTTTTGACTTGTTTGGCTATGATTTAAAAGACTAT
    GATGAATTGTTTGAAGAAAAATTAGGTTTACTTTTAGAGTTAAATAAAACTGAGCGTATT
    ACTTGGTCTGGAAAATATCGTCCAGAACTTAGAAATATGAAAATATTCCCAAGACCAATC
    GATAATATATTGCCAATATGGCGTGCTGTTGGTGGTCCACCTGCAAGTGCTATTAAAGCG
    GGAAAACAAGGTGTGCCAATGATGATTACAACCCTTGGTGGCCCAGCAATGAACTTTAAA
    GGTTCTATAGATGCTTATCGTCAAGCGGCAACTGAAGCAGGTTTCGATGCTTCGCCTAAG
    TCTTTACCAGTAAGTACAGCGAGTCTGTTTTATACAGCTGAAACAACTCAGGATGCTATG
    AGAGAATTTTATCCACATTTGAATACAGGGATGTCATTTATTCGTGGTGTTGGTTATCCG
    AAACAGCAATTTGCTAATTCGTCAGATTATCGAGAAGCGCTAATGGTTGGAAGCCCGCAA
    CAAATTATTGAAAAGATATTGTATCAACACGAGTTGTATGGTCATCAACGTTTTATGGCA
    CAGCTTGATTTTGGCGGTGTGCCATTTGAAAATGTTATGAAGAATATTGAGTTAATTGGC
    AACGACATTATACCGGCGATTAAAAAGCATTTATCAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00319
  • symbol: SAOUHSC_00319
  • description: hypothetical protein
  • length: 353
  • theoretical pI: 6.52142
  • theoretical MW: 39171.5
  • GRAVY: -0.220397

⊟Function[edit | edit source]

  • TIGRFAM:
    probable oxidoreductase, LLM family (TIGR03858; EC 1.-.-.-; HMM-score: 348.7)
    and 20 more
    Unknown function Enzymes of unknown specificity putative luciferase-like monooxygenase, FMN-dependent, CE1758 family (TIGR04036; EC 1.-.-.-; HMM-score: 213.8)
    natural product biosynthesis luciferase-like monooxygenase domain (TIGR04020; EC 1.-.-.-; HMM-score: 91.7)
    Unknown function Enzymes of unknown specificity luciferase family oxidoreductase, group 1 (TIGR03558; EC 1.-.-.-; HMM-score: 62.4)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv2161c family (TIGR03619; EC 1.-.-.-; HMM-score: 58.5)
    Unknown function Enzymes of unknown specificity putative FMN-dependent luciferase-like monooxygenase, KPN_01858 family (TIGR04027; EC 1.-.-.-; HMM-score: 52.9)
    Unknown function Enzymes of unknown specificity luciferase-type oxidoreductase, BA3436 family (TIGR03571; EC 1.-.-.-; HMM-score: 44.8)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 40.5)
    Metabolism Energy metabolism Methanogenesis 5,10-methylenetetrahydromethanopterin reductase (TIGR03555; EC 1.5.98.2; HMM-score: 37.9)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2516 family (TIGR03621; HMM-score: 35.8)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, G6PDH family (TIGR03557; EC 1.-.-.-; HMM-score: 29.3)
    Metabolism Central intermediary metabolism Sulfur metabolism dimethyl sulfone monooxygenase SfnG (TIGR04021; EC 1.14.-.-; HMM-score: 28.5)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2249 family (TIGR03857; HMM-score: 28.4)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_3544 family (TIGR03854; HMM-score: 28)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv3520c family (TIGR03559; HMM-score: 26.9)
    Metabolism Energy metabolism Pentose phosphate pathway glucose-6-phosphate dehydrogenase (coenzyme-F420) (TIGR03554; EC 1.1.98.2; HMM-score: 26.7)
    Unknown function Enzymes of unknown specificity FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family (TIGR03860; EC 1.-.-.-; HMM-score: 23.5)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, CPS_4043 family (TIGR03842; HMM-score: 20.5)
    Unknown function Enzymes of unknown specificity coenzyme F420-dependent oxidoreductase, NP1902A family (TIGR04024; EC 1.14.-.-; HMM-score: 17.7)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, MSMEG_4879 family (TIGR03564; EC 1.-.-.-; HMM-score: 15.9)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2906 family (TIGR03856; HMM-score: 11.8)
  • TheSEED  :
    • Bacterial luciferase family protein
  • PFAM:
    no clan defined Bac_luciferase; Luciferase-like monooxygenase (PF00296; HMM-score: 142.2)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003611
    • TAT(Tat/SPI): 0.0002
    • LIPO(Sec/SPII): 0.00039
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 88194120 NCBI
  • UniProt: Q2G136 UniProt
  • protein Genbank : _
  • RefSeq: YP_498909 NCBI

⊟Protein sequence[edit | edit source]

  • MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEASKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRVFEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERITWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMNFKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRGVGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMKNIELIGNDIIPAIKKHLSK

⊟Peptides[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [4] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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