NCBI date : _
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00071
- pan locus tag?: SAUPAN000911000
- symbol: SAOUHSC_00071
- pan gene symbol?: sirC
- synonym:
- product: lipoprotein SirC
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00071
- symbol: SAOUHSC_00071
- product: lipoprotein SirC
- replicon: chromosome
- strand: -
- coordinates: 76521..77435
- length: 915
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
- Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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901ATGATTGGTTCAAGCTTTATACCATTACAACGCGTACTGATGTACTTTATAAATCCAAAT
GACAGTATGGATCAATTCACTTTAGAAGTATTACGCTTACCTCGCATTACACTTGCGATT
TTAGCAGGTGCCGCACTAGGAATGAGTGGTTTAATGTTGCAAAATGTATTAAAAAATCCA
ATTGCCTCACCTGATATTATCGGTATCACAGGTGGTGCTAGCTTAAGTGCTGTTGTCTTT
ATTGCATTTTTCAGCCATTTAACAATACATTTACTTCCACTATTTGCAGTATTAGGTGGC
GCAGTTGCAATGATGATACTATTAGTGTTTCAAACGAAAGGACAAATACGCCCGACAACA
CTCATAATCATCGGTATTTCGATGCAAACGTTGTTTATTGCGCTTGTCCAAGGATTACTC
ATTACAACGAAGCAATTATCTGCTGCCAAAGCTTATACATGGCTAGTCGGAAGTCTTTAC
GGTGCTACGTTTAAAGATACAATCATTTTGGGTATGGTTATTTTAGCTGTTGTGCCGTTG
TTATTTCTTGTTATACCAAAAATGAAAATATCTATACTTGATGACCCTGTAGCGATTGGC
TTAGGCTTACATGTACAACGTATGAAACTAATCCAATTAATCACTTCTACTATACTCGTA
TCTATGGCAATCAGTTTAGTAGGTAACATTGGGTTTGTCGGTTTAATCGCACCACATATC
GCGAAAACAATCGTTCGCGGAAGTTATGCTAAAAAGTTACTAATGTCAGCAATGATTGGT
GCCATATCAATTGTTATTGCAGACTTAATTGGGCGTACCTTATTCTTGCCTAAAGAAGTG
CCAGCAGGTGTATTTATTGCTGCTTTTGGTGCCCCATTCTTCATATACTTATTATTAACC
GTGAAAAAGTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00071
- symbol: SAOUHSC_00071
- description: lipoprotein SirC
- length: 304
- theoretical pI: 10.7701
- theoretical MW: 32508.6
- GRAVY: 1.14539
⊟Function[edit | edit source]
- TIGRFAM: proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 150.5)and 1 moreTransport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 30.3)
- TheSEED :
- Siderophore staphylobactin ABC transporter, permease protein SirC
- PFAM: Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 275.2)and 2 moreABC-3; ABC 3 transport family (PF00950; HMM-score: 21)ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 12.6)
⊟Structure, modifications & interactions[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- interaction partners:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009926
- TAT(Tat/SPI): 0.001683
- LIPO(Sec/SPII): 0.002536
- predicted transmembrane helices (TMHMM): 8
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MIGSSFIPLQRVLMYFINPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL
⊟Peptides[edit | edit source]
- experimentally validated:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- sigma factors : _
- regulator: Fur* (repression) regulon
Fur* (TF) important in Iron homeostasis; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [1]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit | edit source]
Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)