From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11905 [old locus tag: SACOL2264 ]
  • pan locus tag?: SAUPAN005732000
  • symbol: SACOL_RS11905
  • pan gene symbol?: moaE
  • synonym:
  • product: molybdopterin synthase catalytic subunit

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS11905 [old locus tag: SACOL2264 ]
  • symbol: SACOL_RS11905
  • product: molybdopterin synthase catalytic subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2327278..2327724
  • length: 447
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (2327278..2327724) NCBI
  • BioCyc: SACOL_RS11905 BioCyc
  • MicrobesOnline: see SACOL2264

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAACAATTTGAAATCGTGACAGAACCGATACAAACAGAACAATATCGTGAATTCACT
    ATAAATGAATATCAAGGTGCAGTAGTTGTTTTTACCGGTCATGTTCGCGAATGGACTAAA
    GGCGTCAAAACGGAATATTTAGAATATGAAGCGTATATTCCAATGGCTGAAAAGAAATTG
    GCACAAATTGGAGATGAAATAAATGAAAAATGGCCTGGAACGATAACGAGTATTGTTCAT
    AGAATAGGGCCATTACAAATTTCAGATATCGCTGTATTAATTGCGGTTTCTTCACCGCAT
    CGTAAAGATGCCTATCGAGCAAATGAATATGCAATTGAGCGTATAAAAGAAATTGTTCCG
    ATTTGGAAAAAAGAAATTTGGGAAGATGGTTCAAAATGGCAAGGGCATCAAAAAGGGAAT
    TATGAAGAAGCGAAGAGGGAGGAATAA
    60
    120
    180
    240
    300
    360
    420
    447

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS11905 [old locus tag: SACOL2264 ]
  • symbol: SACOL_RS11905
  • description: molybdopterin synthase catalytic subunit
  • length: 148
  • theoretical pI: 5.35216
  • theoretical MW: 17338.5
  • GRAVY: -0.664189

Function[edit | edit source]

  • reaction:
    EC 2.8.1.12?  ExPASy
    Molybdopterin synthase Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + H2O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
  • TIGRFAM:
  • TheSEED: see SACOL2264
  • PFAM:
    no clan defined MoaE; MoaE protein (PF02391; HMM-score: 120.4)
    and 1 more
    Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 15.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002814
    • TAT(Tat/SPI): 0.00042
    • LIPO(Sec/SPII): 0.000317
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE

Experimental data[edit | edit source]

  • experimentally validated: see SACOL2264
  • protein localization: see SACOL2264
  • quantitative data / protein copy number per cell: see SACOL2264
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]