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m (Text replacement - "* <aureodatabase>protein Genbank</aureodatabase> " to "")
m (Text replacement - "gene Genbank" to "gene RefSeq")
 
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__TOC__
<protect>
<protect>
<aureodatabase>NCBI date</aureodatabase>
<aureodatabase>annotation</aureodatabase>


=Summary=
=Summary=


* <aureodatabase>organism</aureodatabase>
*<aureodatabase>organism</aureodatabase>
* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>pan locus</aureodatabase>
*<aureodatabase>pan locus</aureodatabase>
* <aureodatabase>gene symbol</aureodatabase>
*<aureodatabase>gene symbol</aureodatabase>
* <aureodatabase>pan gene symbol</aureodatabase>
*<aureodatabase>pan gene symbol</aureodatabase>
* <aureodatabase>gene synonyms</aureodatabase>
*<aureodatabase>gene synonyms</aureodatabase>
* <aureodatabase>product</aureodatabase>
*<aureodatabase>product</aureodatabase>
</protect>
</protect>


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==General==
==General==


* <aureodatabase>gene type</aureodatabase>
*<aureodatabase>gene type</aureodatabase>
* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>gene symbol</aureodatabase>
*<aureodatabase>gene symbol</aureodatabase>
* <aureodatabase>product</aureodatabase>
*<aureodatabase>product</aureodatabase>
* <aureodatabase>gene replicon</aureodatabase>
*<aureodatabase>gene replicon</aureodatabase>
* <aureodatabase>strand</aureodatabase>
*<aureodatabase>strand</aureodatabase>
* <aureodatabase>gene coordinates</aureodatabase>
*<aureodatabase>gene coordinates</aureodatabase>
* <aureodatabase>gene length</aureodatabase>
*<aureodatabase>gene length</aureodatabase>
* <aureodatabase>essential</aureodatabase>
*<aureodatabase>essential</aureodatabase>
*<aureodatabase>gene comment</aureodatabase>
</protect>
</protect>


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==Accession numbers==
==Accession numbers==


* <aureodatabase>gene GI</aureodatabase>
*<aureodatabase>gene location</aureodatabase>
* <aureodatabase>gene Genbank</aureodatabase>
*<aureodatabase>gene BioCyc</aureodatabase>
*<aureodatabase>gene MicrobesOnline</aureodatabase>
</protect>
</protect>
   
   
<protect>  
<protect>
==Phenotype==
==Phenotype==
</protect>
</protect>
* Share your knowledge and add information here. [<span class="plainlinks">[http://www.protecs.uni-greifswald.de/aureowiki/index.php?title={{PAGENAMEE}}&action=edit&section=6 edit]</span>]
Share your knowledge and add information here. [<span class="plainlinks">[//aureowiki.med.uni-greifswald.de/index.php?title={{PAGENAMEE}}&veaction=edit&section=6 edit]</span>]


<protect>
<protect>
==DNA sequence==
==DNA sequence==


* <aureodatabase>gene sequence</aureodatabase>
*<aureodatabase>gene sequence</aureodatabase>
</protect>
</protect>


<protect>
<protect>
<aureodatabase>RNA regulated operons</aureodatabase>
</protect>


<protect>
=Protein=
=Protein=
<aureodatabase>protein 3D view</aureodatabase>
<aureodatabase>protein 3D view</aureodatabase>
==General==
==General==


* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>protein symbol</aureodatabase>
*<aureodatabase>protein symbol</aureodatabase>
* <aureodatabase>protein description</aureodatabase>
*<aureodatabase>protein description</aureodatabase>
* <aureodatabase>protein length</aureodatabase>
*<aureodatabase>protein length</aureodatabase>
* <aureodatabase>theoretical pI</aureodatabase>
*<aureodatabase>theoretical pI</aureodatabase>
* <aureodatabase>theoretical MW</aureodatabase>
*<aureodatabase>theoretical MW</aureodatabase>
* <aureodatabase>GRAVY</aureodatabase>
*<aureodatabase>GRAVY</aureodatabase>
</protect>
</protect>


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==Function==
==Function==


* <aureodatabase>protein reaction</aureodatabase>
*<aureodatabase>protein reaction</aureodatabase>
* <aureodatabase>protein TIGRFAM</aureodatabase>
*<aureodatabase>protein TIGRFAM</aureodatabase>
* <aureodatabase>protein TheSeed</aureodatabase>
*<aureodatabase>protein TheSeed</aureodatabase>
* <aureodatabase>protein PFAM</aureodatabase>
*<aureodatabase>protein PFAM</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Structure, modifications & interactions==
==Structure, modifications & cofactors==


* <aureodatabase>protein domains</aureodatabase>
*<aureodatabase>protein domains</aureodatabase>
* <aureodatabase>protein modifications</aureodatabase>
*<aureodatabase>protein modifications</aureodatabase>
* <aureodatabase>protein cofactors</aureodatabase>
*<aureodatabase>protein cofactors</aureodatabase>
* <aureodatabase>protein effectors</aureodatabase>
*<aureodatabase>protein effectors</aureodatabase>
* <aureodatabase>protein partners</aureodatabase>
*<aureodatabase>protein regulated operons</aureodatabase>
</protect>
</protect>


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==Localization==
==Localization==


* <aureodatabase>protein Psortb</aureodatabase>
*<aureodatabase>protein Psortb</aureodatabase>
* <aureodatabase>protein LocateP</aureodatabase>
*<aureodatabase>protein LocateP</aureodatabase>
* <aureodatabase>protein SignalP</aureodatabase>
*<aureodatabase>protein SignalP</aureodatabase>
* <aureodatabase>protein TMHMM</aureodatabase>
*<aureodatabase>protein TMHMM</aureodatabase>
</protect>
</protect>


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==Accession numbers==
==Accession numbers==


* <aureodatabase>protein GI</aureodatabase>
*<aureodatabase>protein GI</aureodatabase>
* <aureodatabase>protein UniProt</aureodatabase>
*<aureodatabase>protein RefSeq</aureodatabase>
* <aureodatabase>protein RefSeq</aureodatabase>
*<aureodatabase>protein UniProt</aureodatabase>
</protect>
</protect>


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==Protein sequence==
==Protein sequence==


* <aureodatabase>protein sequence</aureodatabase>
*<aureodatabase>protein sequence</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Peptides==
==Experimental data==


* <aureodatabase>protein validated peptides</aureodatabase>
*<aureodatabase>protein validated peptides</aureodatabase>
*<aureodatabase>protein validated localization</aureodatabase>
*<aureodatabase>protein validated quantitative data</aureodatabase>
*<aureodatabase>protein partners</aureodatabase>
</protect>
</protect>


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==Operon==
==Operon==


* <aureodatabase>operons</aureodatabase>
*<aureodatabase>operons</aureodatabase>
</protect>
</protect>


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==Regulation==
==Regulation==


* <aureodatabase>sigma factors</aureodatabase>
*<aureodatabase>regulators</aureodatabase>
* <aureodatabase>regulators</aureodatabase>
</protect>
</protect>


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==Transcription pattern==
==Transcription pattern==


* <aureodatabase>expression browser</aureodatabase>
*<aureodatabase>expression browser</aureodatabase>
</protect>
</protect>


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==Protein synthesis (provided by Aureolib)==
==Protein synthesis (provided by Aureolib)==


* <aureodatabase>protein synthesis Aureolib</aureodatabase>
*<aureodatabase>protein synthesis Aureolib</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Stability==
==Protein stability==


* <aureodatabase>protein half-life</aureodatabase>
*<aureodatabase>protein half-life</aureodatabase>
</protect>
</protect>



Latest revision as of 02:16, 11 March 2016

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11235 [old locus tag: SACOL2146 ]
  • pan locus tag?: SAUPAN005495000
  • symbol: SACOL_RS11235
  • pan gene symbol?: mtlF
  • synonym:
  • product: PTS mannitol transporter subunit IICB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS11235 [old locus tag: SACOL2146 ]
  • symbol: SACOL_RS11235
  • product: PTS mannitol transporter subunit IICB
  • replicon: chromosome
  • strand: +
  • coordinates: 2208813..2210351
  • length: 1539
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (2208813..2210351) NCBI
  • BioCyc: SACOL_RS11235 BioCyc
  • MicrobesOnline: see SACOL2146

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCACAAACTGAAGAGAAAAAAGGAATTGGTCGTCGTGTTCAAGCATTTGGATCATTC
    TTAAGTAGTATGATCATGCCAAACATTGGCGCTTTCATCGCTTGGGGTTTTATCGCGGCC
    ATTTTTATAGATAATGGATGGTTACCTAACAAAGATTTAGCTACTTTAGCAGGACCAATG
    ATTACTTATTTAATCCCATTATTGATCGCATTTAGTGGTGGTCGTTTAATTTATGATTTA
    CGTGGTGGTATCATCGCAGCAACAGCAACTATGGGGGTCATCGTTGCATTACCTGATACA
    CCAATGTTACTTGGTGCAATGATTATGGGTCCACTTGTTGGTTGGTTAATGAAGAAGACC
    GACCAATTGATTCAACCTAGAACGCCGCAAGGTTTTGAAATGTTATTTAATAACTTTTCT
    GCTGGTATTTTAGGATTTATCATGACGATTGCAGGATTTAAAATTTTAGCACCACTTATG
    AAATTTATTATGCATATTTTATCAGTGGCAGTTGAAGCATTAGTACATGCACACCTACTT
    CCACTTGTAAGTATTTTAGTAGAGCCTGCAAAAATTGTATTTTTAAACAATGCGATTAAC
    CATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTA
    TACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTT
    GGAAAAGGCACTGCAAAAACAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGGT
    ATTCATGAAATTTATTTCCCATATGTATTAATGCGTCCTTTATTATTTATCGCTGTTATT
    TTAGGTGGAATGACTGGTGTTGCAACTTATCAAGCAACAGGATTTGGATTTAAAAGTCCC
    GCGTCACCAGGTTCATTTATAGTTTACTGTTTAAATGCACCTAGAGGTGAATTCTTGCAC
    ATGTTACTCGGTGTCTTCCTTGCTGCACTTGTATCATTCGTTGTAGCTGCTTTAATTATG
    AAGTTCACTAGAGAACCAAAGCAGGATTTAGAAGCTGCGACAGCTCAAATGGAAAATACT
    AAAGGGAAAAAATCAAGCGTTGCTTCTAAGTTAGTATCTTCTGATAAAAATGTTAATACA
    GAAGAAAATGCTAGTGGTAATGTTAGTGAAACATCTTCATCAGATGATGATCCTGAAGCG
    CTATTGGATAATTACAACACTGAAGATGTTGATGCACACAATTACAATAATATAAATCAT
    GTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGT
    AATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAA
    TTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAA
    CAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA
    GAACTTTTAAATAATCTAAAAAAAGATGATCAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1539

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS11235 [old locus tag: SACOL2146 ]
  • symbol: SACOL_RS11235
  • description: PTS mannitol transporter subunit IICB
  • length: 512
  • theoretical pI: 7.76606
  • theoretical MW: 55095
  • GRAVY: 0.294336

Function[edit | edit source]

  • reaction:
    EC 2.7.1.197?  ExPASy
    Protein-N(pi)-phosphohistidine--D-mannitol phosphotransferase [Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2)
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 588.7)
    Signal transduction Signal transduction PTS PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 588.7)
    and 2 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
  • TheSEED: see SACOL2146
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 59.7)
    and 3 more
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 44)
    Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 16.3)
    no clan defined Ctr; Ctr copper transporter family (PF04145; HMM-score: 13.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009005
    • TAT(Tat/SPI): 0.001102
    • LIPO(Sec/SPII): 0.003247
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIFGKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKKDDQA

Experimental data[edit | edit source]

  • experimentally validated: see SACOL2146
  • protein localization: see SACOL2146
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]