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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07990 [old locus tag: SACOL1569 ]
  • pan locus tag?: SAUPAN004079000
  • symbol: SACOL_RS07990
  • pan gene symbol?: nusB
  • synonym:
  • product: N utilization substance protein B

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07990 [old locus tag: SACOL1569 ]
  • symbol: SACOL_RS07990
  • product: N utilization substance protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 1605121..1605510
  • length: 390
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1605121..1605510) NCBI
  • BioCyc: G1G4B-1680 BioCyc
  • MicrobesOnline: see SACOL1569

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAGTCGTAAAGAATCCCGAGTGCAAGCTTTTCAAACTTTATTTCAATTAGAAATGAAG
    GACAGTGATTTAACGATAAATGAAGCGATAAGCTTTATTAAAGACGATAATCCAGATTTA
    GACTTCGAATTTATTCATTGGCTAGTTTCTGGCGTTAAAGATCACGAACCTGTATTAGAC
    GAGACAATTAGTCCTTATTTAAAAGATTGGACTATTGCACGTTTATTAAAAACGGATCGT
    ATTATTTTAAGAATGGCAACATATGAAATATTACACAGTGATACACCTGCTAAAGTCGTA
    ATGAATGAAGCAGTTGAATTAACAAAACAATTCAGTGATGATGATCATTATAAATTTATA
    AATGGTGTATTGAGTAATATAAAAAAATAA
    60
    120
    180
    240
    300
    360
    390

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS07990 [old locus tag: SACOL1569 ]
  • symbol: SACOL_RS07990
  • description: N utilization substance protein B
  • length: 129
  • theoretical pI: 4.80681
  • theoretical MW: 15061.1
  • GRAVY: -0.351938

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 135.8)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 27.7)
  • TheSEED: see SACOL1569
  • PFAM:
    NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 118.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002999
    • TAT(Tat/SPI): 0.000255
    • LIPO(Sec/SPII): 0.000267
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEFIHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMNEAVELTKQFSDDDHYKFINGVLSNIKK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1569
  • protein localization: see SACOL1569
  • quantitative data / protein copy number per cell: see SACOL1569
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]