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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07250 [old locus tag: SACOL1424 ]
  • pan locus tag?: SAUPAN003798000
  • symbol: SACOL_RS07250
  • pan gene symbol?: pstS
  • synonym:
  • product: phosphate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS07250 [old locus tag: SACOL1424 ]
  • symbol: SACOL_RS07250
  • product: phosphate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1435136..1436119
  • length: 984
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1435136..1436119) NCBI
  • BioCyc: G1G4B-1531 BioCyc
  • MicrobesOnline: see SACOL1424

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAATGGCAATTTGTTGGTACTACAGCTTTAGGTGCAACACTATTATTAGGTGCT
    TGTGGTGGCGGTAATGGTGGCAGTGGTAATAGTGATTTAAAAGGGGAAGCTAAAGGTGAT
    GGCTCATCAACAGTAGCACCAATTGTGGAGAAATTAAATGAAAAATGGGCTCAAGATCAC
    TCGGATGCTAAAATCTCAGCAGGACAAGCTGGTACAGGTGCTGGTTTCCAAAAATTCATT
    GCAGGAGATATCGACTTCGCTGATGCTTCTAGACCAATTAAAGATGAAGAGAAGCAAAAA
    TTACAAGATAAGAATATCAAATACAAAGAATTCAAAATTGCGCAAGATGGTGTAACGGTT
    GCTGTAAATAAAGAAAATGATTTTGTAGATGAATTAGACAAACAGCAATTAAAAGCAATT
    TATTCTGGAAAAGCTAAAACATGGAAAGATGTTAATAGTAAATGGCCAGATAAAAAAATA
    AATGCTGTATCACCAAACTCAAGTCATGGTACTTATGACTTCTTTGAAAATGAAGTAATG
    AATAAAGAAGATATTAAAGCAGAAAAAAATGCTGATACAAATGCTATCGTTTCTTCTGTA
    ACGAAAAACAAAGAGGGAATCGGATACTTTGGATATAACTTCTACGTACAAAATAAAGAT
    AAATTAAAAGAAGTTAAAATCAAAGATGAAAATGGTAAAGCAACAGAGCCTACGAAAAAA
    ACAATTCAAGATAACTCTTATGCATTAAGTAGACCATTATTCATTTATGTAAATGAAAAA
    GCATTGAAAGATAATAAAGTAATGTCAGAATTTATCAAATTCGTCTTAGAAGATAAAGGT
    AAAGCAGCTGAAGAAGCTGGATATGTAGCAGCACCAGAGAAAACATACAAATCACAATTA
    GATGATTTAAAAGCATTTATTGATAAAAATCAAAAATCAGACGACAAGAAATCTGATGAT
    AAAAAGTCTGAAGACAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS07250 [old locus tag: SACOL1424 ]
  • symbol: SACOL_RS07250
  • description: phosphate-binding protein
  • length: 327
  • theoretical pI: 8.71151
  • theoretical MW: 36117.2
  • GRAVY: -0.870031

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 344.1)
    and 2 more
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, phosphate-binding protein PstS (TIGR00975; HMM-score: 65)
    phosphate ABC transporter substrate-binding protein (TIGR04505; HMM-score: 50.1)
  • TheSEED: see SACOL1424
  • PFAM:
    PBP (CL0177) PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 137.8)
    and 1 more
    PBP_like; PBP superfamily domain (PF12727; HMM-score: 60.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000881
    • TAT(Tat/SPI): 0.0001
    • LIPO(Sec/SPII): 0.998765
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9991
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSSTVAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQDKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKINAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQNKDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVLEDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1424
  • protein localization: see SACOL1424
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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