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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05175 [old locus tag: SACOL1013 ]
  • pan locus tag?: SAUPAN003180000
  • symbol: SACOL_RS05175
  • pan gene symbol?: mgtE
  • synonym:
  • product: magnesium transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05175 [old locus tag: SACOL1013 ]
  • symbol: SACOL_RS05175
  • product: magnesium transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 1020124..1021509
  • length: 1386
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1020124..1021509) NCBI
  • BioCyc: SACOL_RS05175 BioCyc
  • MicrobesOnline: see SACOL1013

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAATGAACACAGATGAAAAAGAGCGTGTTCAAGAGGAATTATACGACCAAACTTTA
    TTAGATCAATATTTAGAAAATGATGATATTGATCAATTTAGAGATGAATTTCTAGCATTA
    CACACATATGAACAAAGTGAGTATTTTGAAGATACTACCGATGAAAATAGACAAAAGATT
    TTTCAATATTTATCACCTGAAGAAGTTGCAAATTTCTTTGATCAATTAGATATTGATGAC
    GATGAATATGAGTTGCTATTTGATAAGATGAATGCGACATACGCAAGTCACATATTAGAA
    GAAATGTCATACGACAATGCAGTAGATATTTTAAATGAGTTGACTAAACCAAAAGTTGCT
    AGTCTTTTAACATTGATGAATAAAGATGACGCGAATGAAATCAAAGCATTACTTCACTAT
    GATGAGGATACGGCCGGCGGTATTATGACGACGGAGTATTTATCACTTAAAGCGCATACG
    CCTGTTAAAGAAGCATTATTATTGGTCAAAGCGCAAGCACCAGACGCAGAAACAATATAT
    GTTATATTTGTCGTTGATGATGATGGTAAATTAGTAGGTGTTTTATCGCTAAGAGATTTA
    ATTGTAGCTGAAAATGATGCTTATATTGAAGATATTATGAATGAACGTGTCATTAGTGTG
    AATGTAGCAGACGACCAAGAAGATGTTGCTCAAGTTATGAGAGACTATGATTTCATGGCT
    GTACCTGTTATAGATTACCAAGAACATTTGCTTGGTATCATCACGATTGATGATATTTTA
    GACGTTATGGATGAAGAGGCTAGTGAAGACTACTCTCGTTTAGCCGGGGTATCAGATATC
    GATTCGACTAATGATTCAATCATTAAAACAGCATTAAAACGTTTACCATGGTTGATTATT
    TTAACATTTTTAGGAATGATTACTGCGACAATTTTAGGGAGATTCGAAAAAACATTAGAA
    AATGTAGCGCTACTCGCAGCGTTTATTCCTATTATTAGTGGTATGTCAGGAAATTCAGGT
    ACACAATCTTTAGCCGTTTCAGTTCGTAACATTACGACAGGGGAAATTAATGAGCAAAGT
    AAATTTAGAATTGCATTAAGAGAAGCAGGAAGTGGTGTATTATCGGGTGTTGTATGTTCA
    ACAATATTATTTACAATTATTGTTGCAATATATCATCAGCCACTTTTAGCATTAATCGTT
    GCAGGAAGTTTAACTTGTGCGATGACGGTGGGGACGTTTGTAGGTTCGATGATTCCATTA
    TTGATGAATAAATTAAATATCGATCCAGCAGTGGCTAGTGGACCATTTATTACAACAATT
    AATGATATTATTAGTATGTTGATTTATTTTGGTTTAGCTACATCATTTATGGCTTACTTA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS05175 [old locus tag: SACOL1013 ]
  • symbol: SACOL_RS05175
  • description: magnesium transporter
  • length: 461
  • theoretical pI: 3.87276
  • theoretical MW: 51435.2
  • GRAVY: 0.13731

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 405.1)
    and 6 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 39.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 32.2)
    Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 18.5)
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 15.6)
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 13.5)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 12.7)
  • TheSEED: see SACOL1013
  • PFAM:
    no clan defined MgtE; Divalent cation transporter (PF01769; HMM-score: 97.2)
    FliG (CL0436) MgtE_N; MgtE intracellular N domain (PF03448; HMM-score: 84.8)
    and 1 more
    no clan defined CBS; CBS domain (PF00571; HMM-score: 72)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002358
    • TAT(Tat/SPI): 0.00418
    • LIPO(Sec/SPII): 0.000491
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFGLATSFMAYLI

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1013
  • protein localization: see SACOL1013
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]