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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04180 [old locus tag: SACOL0813 ]
  • pan locus tag?: SAUPAN002659000
  • symbol: SACOL_RS04180
  • pan gene symbol?: comFA
  • synonym:
  • product: comf operon protein 1

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS04180 [old locus tag: SACOL0813 ]
  • symbol: SACOL_RS04180
  • product: comf operon protein 1
  • replicon: chromosome
  • strand: +
  • coordinates: 836220..837302
  • length: 1083
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (836220..837302) NCBI
  • BioCyc: SACOL_RS04180 BioCyc
  • MicrobesOnline: see SACOL0813

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATAT
    TATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGA
    GCCATTAGAAAGAGACAAACGATTTTGTTATATGCCGTAACAGGTGCAGGTAAGACAGAA
    ATGATGTTTCAAGGCATTCAATATGCAAGAATACAGGGAGATAATATAGCTATTGTGTCA
    CCACGTGTAGATGTTGTTGTAGAAATTAGTAAACGTATTAAAGACGCATTTCTTAATGAA
    GATATAGACATACTACACCAGCAATCAAGACAACAATTTGAAGGGCATTTTGTTGTATGC
    ACAGTGCATCAACTTTACCGATTCAAACAGCACTTTGATACTATTTTTATTGATGAAGTC
    GATGCCTTTCCTTTATCAATGGATAAAAATTTACAACAAGCATTGAAGTCATCTTCTAAA
    GTTGAACATGCAACAATTTATATGACAGCAACACCACCGAAACAACTTCTGTCAGAGATT
    CCCCACGAAAATATAATTAAATTGCCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCT
    AAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTA
    CAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATG
    ATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAG
    GATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTT
    ACTACGACAATATTAGAACGTGGATTTACAATGGCAAATTTGGATGTTGTTGTTATCGAT
    GCACATCAATATACTCAAGAGGCTTTAATACAAATTGCTGGACGTGTTGGACGAAAATTA
    GAATGTCCTACTGGAAAAGTATTGTTTTTTCATGAAGGGGTAAGTATGAATATGATTCAA
    GCTAAAAAAGAGATTCAAAGGATGAACAAATTAGCATTAAAAAGAGGTTGGATTGATGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1083

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS04180 [old locus tag: SACOL0813 ]
  • symbol: SACOL_RS04180
  • description: comf operon protein 1
  • length: 360
  • theoretical pI: 9.83425
  • theoretical MW: 42111.4
  • GRAVY: -0.346111

Function[edit | edit source]

  • TIGRFAM:
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 50.8)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 28.8)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 19.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 19.5)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 18.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 13.4)
  • TheSEED: see SACOL0813
  • PFAM:
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 44.3)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 44)
    and 10 more
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 25.4)
    Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 20.9)
    no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 19.1)
    P-loop_NTPase (CL0023) Helicase_RecD; Helicase (PF05127; HMM-score: 18.6)
    AAA_19; AAA domain (PF13245; HMM-score: 17.6)
    AAA_30; AAA domain (PF13604; HMM-score: 16.1)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.9)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 13.4)
    AAA_24; AAA domain (PF13479; HMM-score: 12.1)
    Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 10.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010697
    • TAT(Tat/SPI): 0.000822
    • LIPO(Sec/SPII): 0.000791
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRKRQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDIDILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSKVEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]