From AureoWiki
Revision as of 12:18, 11 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03560 [old locus tag: SACOL0692 ]
  • pan locus tag?: SAUPAN002511000
  • symbol: SACOL_RS03560
  • pan gene symbol?:
  • synonym:
  • product: membrane protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03560 [old locus tag: SACOL0692 ]
  • symbol: SACOL_RS03560
  • product: membrane protein
  • replicon: chromosome
  • strand: -
  • coordinates: 716326..717078
  • length: 753
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (716326..717078) NCBI
  • BioCyc: G1G4B-741 BioCyc
  • MicrobesOnline: see SACOL0692

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAATTATTTATCAAACTTTTTTAATACGACAATTCAACTTAATATATACATTATCGTT
    ATTGTTGCAGCTTGTTATATTGCTATTCATCAATATAGACATAAGCCTGTTTTGAACTAT
    TTAGATGTCATTTTAAACTATATCCCTGTTTTAACACATGAATTTGGTCATGTACTGTTT
    AATAAACTCGCTGGTGGCCGTGCAAAAGATTTAGTCATTGTAACAAGTCCACGAGAGCGA
    CAACAGACATTACAACAAGGATTCGCAATTACACAATCTAGACATCTAGCAGGCCAATGG
    CTTACAACGATTGGTGGTTATTTTATGCCACCTATTATGTTATTGATAGGTTTAGCGTCG
    AGCCATTATCAGATACCTAGTTTCTTTATATTTACCTATTTATTGATATTTATTTATTTT
    TTAATTTTAACATCTAGAAAAGGCTCACCCATTGTTGTTATAACTTTAATTTCTATCATG
    TTGTATTTTATATTAAAAGATGAAAATATAGTAGAAATTCAACTACTTGTCACAATGAGT
    TATCAATATATTTTAGGTGTATTATTAGGCGAAGTTCTACAATCTAGTTGGACGATTGCT
    AAATTGACTTTCCAGCGTCCGAGTCCACAGTGGGATGGTAGTGCACTTAAAGAACTCAGT
    CATGTACCTATCTTCATTTATAGTACAATCTGGATTATCTTCAATCTTTATGCAGTGAAT
    ATTTTACTCAAATATACTCACTTGATAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS03560 [old locus tag: SACOL0692 ]
  • symbol: SACOL_RS03560
  • description: membrane protein
  • length: 250
  • theoretical pI: 9.44382
  • theoretical MW: 28795.9
  • GRAVY: 0.606

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 13.5)
  • TheSEED: see SACOL0692
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M50B; Peptidase M50B-like (PF13398; HMM-score: 152.4)
    and 2 more
    no clan defined Otopetrin; Otopetrin (PF03189; HMM-score: 16.5)
    Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
    • SP(Sec/SPI): 0.008228
    • TAT(Tat/SPI): 0.000365
    • LIPO(Sec/SPII): 0.515518
    • Cleavage Site: CS pos: 24-25. VAA-CY. Pr: 0.5202
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVILNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLTTIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIMLYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKELSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL0692
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]