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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01250 [old locus tag: SACOL0247 ]
  • pan locus tag?: SAUPAN001141000
  • symbol: SACOL_RS01250
  • pan gene symbol?: lrgA
  • synonym:
  • product: antiholin-like protein LrgA

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS01250 [old locus tag: SACOL0247 ]
  • symbol: SACOL_RS01250
  • product: antiholin-like protein LrgA
  • replicon: chromosome
  • strand: +
  • coordinates: 288034..288477
  • length: 444
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (288034..288477) NCBI
  • BioCyc: SACOL_RS01250 BioCyc
  • MicrobesOnline: see SACOL0247

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGTCGTGAAACAACAAAAAGACGCATCAAAACCAGCACACTTTTTTCACCAAGTCATT
    GTAATTGCTTTAGTACTCTTTGTATCGAAAATAATTGAATCATTTATGCCAATTCCTATG
    CCTGCATCAGTAATCGGTTTAGTATTATTATTTGTATTATTATGTACTGGTGCTGTTAAG
    TTAGGCGAAGTCGAAAAAGTAGGAACGACACTAACAAATAACATTGGCTTACTCTTCGTA
    CCAGCCGGTATCTCAGTTGTTAACTCTTTAGGTGTCATTAGCCAAGCACCATTTTTAATC
    ATTGGACTAATAATCGTCTCAACAATACTATTACTTATTTGTACTGGCTATGTCACACAA
    ATTATTATGAAAGTTACTTCGAGATCTAAAGGTGACAAAGTCACAAAAAAGATCAAAATA
    GAGGAGGCACAAGCTCATGATTAA
    60
    120
    180
    240
    300
    360
    420
    444

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS01250 [old locus tag: SACOL0247 ]
  • symbol: SACOL_RS01250
  • description: antiholin-like protein LrgA
  • length: 147
  • theoretical pI: 10.0032
  • theoretical MW: 15780.1
  • GRAVY: 0.904082

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SACOL0247
  • PFAM:
    no clan defined LrgA; LrgA family (PF03788; HMM-score: 85.2)
    and 2 more
    SieB; Super-infection exclusion protein B (PF14163; HMM-score: 14.8)
    GT-C (CL0111) YfhO; Bacterial membrane protein YfhO (PF09586; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01492
    • TAT(Tat/SPI): 0.000397
    • LIPO(Sec/SPII): 0.008053
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASVIGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIGLIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]