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NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2126 [new locus tag: SACOL_RS11125 ]
  • pan locus tag?: SAUPAN005430000
  • symbol: luxS
  • pan gene symbol?: luxS
  • synonym:
  • product: S-ribosylhomocysteinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2126 [new locus tag: SACOL_RS11125 ]
  • symbol: luxS
  • product: S-ribosylhomocysteinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2187898..2188368
  • length: 471
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3237462 NCBI
  • gene Genbank : _

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACAAAAATGAATGTTGAAAGTTTCAATTTAGATCATACTAAGGTGGTTGCCCCATTT
    ATTAGATTAGCGGGAACGATGGAAGGATTAAACGGAGATGTCATTCACAAATACGACATT
    CGTTTCAAACAACCAAACAAAGAACATATGGATATGCCCGGACTACATTCATTAGAACAT
    TTAATGGCTGAAAATATTAGAAATCATAGTGACAAAGTTGTTGATTTAAGTCCTATGGGT
    TGCCAAACTGGTTTCTATGTATCATTTATTAATCATGATAATTATGATGATGTATTAAAT
    ATTGTTGAAGCAACTTTAAATGATGTGCTAAATGCTACTGAAGTGCCTGCTTGTAATGAA
    GTACAATGTGGCTGGGCAGCAAGTCATTCATTAGAAGGTGCTAAAACTATCGCTCAAGCA
    TTTCTAGACAAACGAAACGAATGGCATGATGTTTTCGGTACAGGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    471

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2126 [new locus tag: SACOL_RS11125 ]
  • symbol: LuxS
  • description: S-ribosylhomocysteinase
  • length: 156
  • theoretical pI: 5.45928
  • theoretical MW: 17513.7
  • GRAVY: -0.355128

⊟Function[edit | edit source]

  • reaction:
    EC 4.4.1.21?  ExPASy
    S-ribosylhomocysteine lyase S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
  • TIGRFAM:
  • TheSEED  :
    • Autoinducer-2 production protein LuxS
    • S-ribosylhomocysteine lyase (EC 4.4.1.21)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  S-ribosylhomocysteine lyase (EC 4.4.1.21)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  S-ribosylhomocysteine lyase (EC 4.4.1.21)
  • PFAM:
    Peptidase_ME (CL0094) LuxS; S-Ribosylhomocysteinase (LuxS) (PF02664; HMM-score: 186.4)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Fe cation
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005651
    • TAT(Tat/SPI): 0.000362
    • LIPO(Sec/SPII): 0.001327
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQPNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIVEATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK

⊟Peptides[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: 23.14 h [1]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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