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m (Text replacement - "gene Genbank" to "gene RefSeq") |
m (Text replacement - "* <aureodatabase>protein Genbank</aureodatabase> " to "") |
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__TOC__ | |||
<protect> | <protect> | ||
<aureodatabase> | <aureodatabase>annotation</aureodatabase> | ||
=Summary= | =Summary= | ||
* <aureodatabase>organism</aureodatabase> | *<aureodatabase>organism</aureodatabase> | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>pan locus</aureodatabase> | *<aureodatabase>pan locus</aureodatabase> | ||
* <aureodatabase>gene symbol</aureodatabase> | *<aureodatabase>gene symbol</aureodatabase> | ||
* <aureodatabase>pan gene symbol</aureodatabase> | *<aureodatabase>pan gene symbol</aureodatabase> | ||
* <aureodatabase>gene synonyms</aureodatabase> | *<aureodatabase>gene synonyms</aureodatabase> | ||
* <aureodatabase>product</aureodatabase> | *<aureodatabase>product</aureodatabase> | ||
</protect> | </protect> | ||
Line 24: | Line 25: | ||
==General== | ==General== | ||
* <aureodatabase>gene type</aureodatabase> | *<aureodatabase>gene type</aureodatabase> | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>gene symbol</aureodatabase> | *<aureodatabase>gene symbol</aureodatabase> | ||
* <aureodatabase>product</aureodatabase> | *<aureodatabase>product</aureodatabase> | ||
* <aureodatabase>gene replicon</aureodatabase> | *<aureodatabase>gene replicon</aureodatabase> | ||
* <aureodatabase>strand</aureodatabase> | *<aureodatabase>strand</aureodatabase> | ||
* <aureodatabase>gene coordinates</aureodatabase> | *<aureodatabase>gene coordinates</aureodatabase> | ||
* <aureodatabase>gene length</aureodatabase> | *<aureodatabase>gene length</aureodatabase> | ||
* <aureodatabase>essential</aureodatabase> | *<aureodatabase>essential</aureodatabase> | ||
*<aureodatabase>gene comment</aureodatabase> | |||
</protect> | </protect> | ||
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==Accession numbers== | ==Accession numbers== | ||
* <aureodatabase>gene GI</aureodatabase> | *<aureodatabase>gene GI</aureodatabase> | ||
* <aureodatabase>gene RefSeq</aureodatabase> | *<aureodatabase>gene RefSeq</aureodatabase> | ||
*<aureodatabase>gene BioCyc</aureodatabase> | |||
*<aureodatabase>gene MicrobesOnline</aureodatabase> | |||
</protect> | </protect> | ||
<protect> | <protect> | ||
==Phenotype== | ==Phenotype== | ||
</protect> | </protect> | ||
Share your knowledge and add information here. [<span class="plainlinks">[//aureowiki.med.uni-greifswald.de/index.php?title={{PAGENAMEE}}&veaction=edit§ion=6 edit]</span>] | |||
<protect> | <protect> | ||
==DNA sequence== | ==DNA sequence== | ||
* <aureodatabase>gene sequence</aureodatabase> | *<aureodatabase>gene sequence</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
<aureodatabase>RNA regulated operons</aureodatabase> | |||
</protect> | |||
<protect> | |||
=Protein= | =Protein= | ||
<aureodatabase>protein 3D view</aureodatabase> | <aureodatabase>protein 3D view</aureodatabase> | ||
==General== | ==General== | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>protein symbol</aureodatabase> | *<aureodatabase>protein symbol</aureodatabase> | ||
* <aureodatabase>protein description</aureodatabase> | *<aureodatabase>protein description</aureodatabase> | ||
* <aureodatabase>protein length</aureodatabase> | *<aureodatabase>protein length</aureodatabase> | ||
* <aureodatabase>theoretical pI</aureodatabase> | *<aureodatabase>theoretical pI</aureodatabase> | ||
* <aureodatabase>theoretical MW</aureodatabase> | *<aureodatabase>theoretical MW</aureodatabase> | ||
* <aureodatabase>GRAVY</aureodatabase> | *<aureodatabase>GRAVY</aureodatabase> | ||
</protect> | </protect> | ||
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==Function== | ==Function== | ||
* <aureodatabase>protein reaction</aureodatabase> | *<aureodatabase>protein reaction</aureodatabase> | ||
* <aureodatabase>protein TIGRFAM</aureodatabase> | *<aureodatabase>protein TIGRFAM</aureodatabase> | ||
* <aureodatabase>protein TheSeed</aureodatabase> | *<aureodatabase>protein TheSeed</aureodatabase> | ||
* <aureodatabase>protein PFAM</aureodatabase> | *<aureodatabase>protein PFAM</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
==Structure, modifications & | ==Structure, modifications & cofactors== | ||
* <aureodatabase>protein domains</aureodatabase> | *<aureodatabase>protein domains</aureodatabase> | ||
* <aureodatabase>protein modifications</aureodatabase> | *<aureodatabase>protein modifications</aureodatabase> | ||
* <aureodatabase>protein cofactors</aureodatabase> | *<aureodatabase>protein cofactors</aureodatabase> | ||
* <aureodatabase>protein effectors</aureodatabase> | *<aureodatabase>protein effectors</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein regulated operons</aureodatabase> | ||
</protect> | </protect> | ||
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==Localization== | ==Localization== | ||
* <aureodatabase>protein Psortb</aureodatabase> | *<aureodatabase>protein Psortb</aureodatabase> | ||
* <aureodatabase>protein LocateP</aureodatabase> | *<aureodatabase>protein LocateP</aureodatabase> | ||
* <aureodatabase>protein SignalP</aureodatabase> | *<aureodatabase>protein SignalP</aureodatabase> | ||
* <aureodatabase>protein TMHMM</aureodatabase> | *<aureodatabase>protein TMHMM</aureodatabase> | ||
</protect> | </protect> | ||
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==Accession numbers== | ==Accession numbers== | ||
* <aureodatabase>protein GI</aureodatabase> | *<aureodatabase>protein GI</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein RefSeq</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein UniProt</aureodatabase> | ||
</protect> | </protect> | ||
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==Protein sequence== | ==Protein sequence== | ||
* <aureodatabase>protein sequence</aureodatabase> | *<aureodatabase>protein sequence</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
== | ==Experimental data== | ||
* <aureodatabase>protein validated peptides</aureodatabase> | *<aureodatabase>protein validated peptides</aureodatabase> | ||
*<aureodatabase>protein validated localization</aureodatabase> | |||
*<aureodatabase>protein validated quantitative data</aureodatabase> | |||
*<aureodatabase>protein partners</aureodatabase> | |||
</protect> | </protect> | ||
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==Operon== | ==Operon== | ||
* <aureodatabase>operons</aureodatabase> | *<aureodatabase>operons</aureodatabase> | ||
</protect> | </protect> | ||
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==Regulation== | ==Regulation== | ||
*<aureodatabase>regulators</aureodatabase> | |||
* <aureodatabase>regulators</aureodatabase> | |||
</protect> | </protect> | ||
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==Transcription pattern== | ==Transcription pattern== | ||
* <aureodatabase>expression browser</aureodatabase> | *<aureodatabase>expression browser</aureodatabase> | ||
</protect> | </protect> | ||
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==Protein synthesis (provided by Aureolib)== | ==Protein synthesis (provided by Aureolib)== | ||
* <aureodatabase>protein synthesis Aureolib</aureodatabase> | *<aureodatabase>protein synthesis Aureolib</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
== | ==Protein stability== | ||
* <aureodatabase>protein half-life</aureodatabase> | *<aureodatabase>protein half-life</aureodatabase> | ||
</protect> | </protect> | ||
Latest revision as of 12:15, 11 March 2016
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1966 [new locus tag: SACOL_RS10280 ]
- pan locus tag?: SAUPAN004934000
- symbol: pcrA
- pan gene symbol?: pcrA
- synonym:
- product: ATP-dependent DNA helicase PcrA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1966 [new locus tag: SACOL_RS10280 ]
- symbol: pcrA
- product: ATP-dependent DNA helicase PcrA
- replicon: chromosome
- strand: -
- coordinates: 2028699..2030891
- length: 2193
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238182 NCBI
- RefSeq: YP_186790 NCBI
- BioCyc: see SACOL_RS10280
- MicrobesOnline: 913444 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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2161ATGAATGCGTTATTAAATCATATGAATACAGAGCAAAGTGAAGCTGTAAAGACAACAGAA
GGACCATTGTTAATTATGGCAGGTGCTGGTTCAGGGAAGACACGTGTTTTAACACATAGA
ATTGCTTATTTATTAGACGAAAAAGATGTCTCACCATACAATGTTTTGGCTATTACTTTT
ACAAATAAAGCTGCAAGAGAAATGAAAGAACGTGTTCAAAAATTAGTAGGTGATCAAGCA
GAAGTTATTTGGATGTCAACATTCCACTCAATGTGTGTTCGTATTTTACGTCGTGATGCA
GATCGAATTGGTATAGAACGCAATTTTACGATAATTGATCCTACAGACCAAAAATCTGTT
ATTAAAGACGTCTTAAAAAATGAAAATATTGATAGTAAAAAGTTTGAACCTCGTATGTTT
ATCGGTGCGATCAGTAATTTGAAAAATGAACTTAAAACACCTGCAGATGCTCAAAAAGAA
GCCACAGATTATCACTCGCAAATGGTAGCAACGGTTTATAGTGGATATCAACGCCAATTG
TCACGTAATGAAGCGTTAGATTTTGATGACCTTATTATGACAACGATTAACTTATTTGAG
CGTGTACCAGAAGTTCTAGAATATTATCAGAACAAATTCCAATATATTCATGTAGATGAG
TATCAAGATACTAATAAAGCACAATACACATTAGTTAAATTATTAGCAAGTAAGTTTAAA
AACTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATGGTTGGCGTGGTGCTGATATT
CAAAATATCTTATCATTTGAAAAAGACTATCCAGAAGCGAATACAATCTTTTTAGAGCAA
AATTATCGTTCGACGAAAACGATTTTAAATGCGGCTAACGAAGTGATTAAAAATAATTCT
GAACGTAAGCCAAAAGGACTGTGGACTGCAAATACGAATGGTGAGAAAATTCATTACTAT
GAAGCAATGACGGAACGTGATGAAGCGGAATTTGTAATACGAGAAATTATGAAGCATCAA
CGTAATGGTAAGAAATATCAAGATATGGCAATTTTATATAGAACGAATGCACAATCACGT
GTACTTGAGGAAACATTCATGAAATCTAATATGCCATACACAATGGTTGGTGGCCAAAAG
TTCTATGACCGTAAAGAAATCAAAGATTTATTAAGTTATTTGCGTATAATTGCCAATAGT
AATGATGACATTAGTTTGCAACGTATTATTAATGTTCCAAAAAGAGGTGTAGGTCCTTCA
TCTGTTGAAAAAGTTCAAAACTATGCACTTCAAAACAATATCAGTATGTTTGATGCACTT
GGAGAAGCTGATTTTATCGGATTGTCAAAAAAGGTGACACAAGAGTGTCTTAATTTTTAC
GAGTTAATCCAAAGCTTGATAAAAGAACAAGAATTTTTAGAAATTCATGAAATAGTTGAT
GAAGTATTACAAAAGTCTGGCTATCGAGAAATGCTTGAACGTGAAAATACATTAGAATCT
CGAAGTAGATTAGAAAACATCGATGAATTTATGTCAGTACCAAAAGACTATGAGGAAAAT
ACCCCATTAGAAGAACAGTCATTAATTAACTTTTTAACGGATTTATCGTTAGTAGCTGAT
ATTGATGAGGCAGATACTGAAAATGGCGTAACACTAATGACGATGCACTCGGCTAAGGGC
CTTGAATTTCCAATTGTCTTTATAATGGGGATGGAAGAATCTTTATTCCCACATATTAGA
GCGATTAAGAGTGAAGATGATCATGAAATGCAAGAAGAACGTCGTATTTGTTATGTAGCA
ATTACAAGGGCTGAAGAGGTGTTATATATCACTCATGCGACATCAAGAATGTTATTTGGT
CGCCCTCAGTCAAATATGCCATCCAGATTTTTAAAGGAAATTCCAGAATCACTATTAGAA
AATCATTCAAGTGGCAAACGACAAACGATACAACCTAAGGCAAAACCTTTTGCTAAACGC
GGATTTAGTCAACGAACAACGTCAACGAAAAAACAAGTATTGTCATCTGATTGGAATGTA
GGTGACAAAGTGATGCATAAAGCCTGGGGAGAAGGCATGGTGAGTAATGTAAACGAGAAA
AATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCG
CAATTTGCACCAATTGAAAAAAAGGAGGATTAA60
120
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1966 [new locus tag: SACOL_RS10280 ]
- symbol: PcrA
- description: ATP-dependent DNA helicase PcrA
- length: 730
- theoretical pI: 5.63681
- theoretical MW: 84073
- GRAVY: -0.546438
⊟Function[edit | edit source]
- reaction: EC 3.6.4.12? ExPASyDNA helicase ATP + H2O = ADP + phosphateEC 3.6.1.-? ExPASy
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 1189.2)and 5 moreDNA metabolism DNA replication, recombination, and repair DNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 737.1)DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 661.4)DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 206.9)DNA metabolism DNA replication, recombination, and repair double-strand break repair helicase AddA (TIGR02784; HMM-score: 158.8)DNA metabolism DNA replication, recombination, and repair exodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 156.8)
- TheSEED :
- ATP-dependent DNA helicase UvrD/PcrA
- PFAM: P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 292.8)UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 267.2)and 9 moreAAA_19; AAA domain (PF13245; HMM-score: 122.7)Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 37.9)UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 37.5)AAA_30; AAA domain (PF13604; HMM-score: 33.2)Exonuc_V_gamma; Exodeoxyribonuclease V, gamma subunit (PF04257; HMM-score: 20.2)AAA_11; AAA domain (PF13086; HMM-score: 18.1)ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)Vps51 (CL0295) Vps51; Vps51/Vps67 (PF08700; HMM-score: 14.4)P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 12.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00559
- TAT(Tat/SPI): 0.001019
- LIPO(Sec/SPII): 0.001743
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 158 [4]
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: camS < ligA < pcrA < SACOL1967
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ 5.0 5.1 5.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Sandy Dubaele, Christophe Martin, Jacqueline Bohn, Patrick Chène
Biochemical study of recombinant PcrA from Staphylococcus aureus for the development of screening assays.
J Biochem Mol Biol: 2007, 40(1);7-14
[PubMed:17244476] [WorldCat.org] [DOI] (P p)