NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
- pan locus tag?: SAUPAN004850000
- symbol: mutY
- pan gene symbol?: mutY
- synonym:
- product: A/G-specific adenine glycosylase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
- symbol: mutY
- product: A/G-specific adenine glycosylase
- replicon: chromosome
- strand: -
- coordinates: 1990591..1991628
- length: 1038
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237527 NCBI
- RefSeq: YP_186751 NCBI
- BioCyc: see SACOL_RS10070
- MicrobesOnline: 913405 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGTATCAGCAGTCTAGTTTTAAAGAAAATTTGATACACTGGTTTGATGAAAATCAAAGA
GAAATGCCATGGCGACAAACGACCAATCCGTACTATATTTGGTTAAGTGAGGTCATGTTA
CAACAGACACAAGTTAAAACAGTTATTGACTATTATCATCGTTTTGTTGAACGATTTCCA
ACTGTGGAAGTTTTGAGTCAAGCTTCGGAAGATGAAGTGTTAAAGTATTGGGAAGGCTTA
GGATATTATAGTAGAGCTCGAAATTTTCATACGGCGATTAAAGAAGTTCATGATAAATAT
GAAGGCTTAGTTCCCAAAGATCCTGATCAATTTAAAGCGTTAAAAGGTGTTGGCCCATAC
ACACAAGCTGCAGTAATGAGCATTGCATATAATGTACCACTAGCAACTGTAGACGGAAAT
GTTTTTCGAGTATGGTCTAGATTGAATGATGACTATCGAGATATTAAATTACAATCGACG
AGGAAATCGTACGAACAAGAATTATTACCGTATGTTACTACAGAAGCTGGAACGTTTAAT
CAAGCTATGATGGAATTAGGGGCGTTAATTTGTACACCAAAAAATCCATTATGCTTATTT
TGTCCAGTTCAAGAAAACTGTGAGGCATTTGATAAAGGTACATTTGAAAAGTTACCTGTT
AAATCTAAAAATGTAAGTAAAAAAGTAATTGAGCAATCCGTCTTTTTAATTAGAAATAAT
CAAGGTCAATATCTGTTGCAAAAGCGTAGCGAAAAATTATTGCATGGTATGTGGCAATTT
CCTATGTTTGAAAGTGAGCATGCTAGACGTAAAATGACTGAAAAAATCGGTCATGACATT
CAACCAGTAGAAACACCTATTTTTGAGTTGAAGCATCAATTTACTCATTTAACATGGAAG
ATTAAAGTATACGCCGTTTCAGGAGCAATTAACATTGAAACATTACCAGATGACATGATT
TGGTTTGATTTAAGTGATCGAGATCAATATACATTCCCTGTACCTATGTCAAAGATTTAT
CAATTTATAAATGGGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
- symbol: MutY
- description: A/G-specific adenine glycosylase
- length: 345
- theoretical pI: 6.79462
- theoretical MW: 40623.2
- GRAVY: -0.495652
⊟Function[edit | edit source]
- reaction: EC 3.2.2.-? ExPASy
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair A/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 328.3)and 1 moreDNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 74.1)
- TheSEED :
- A/G-specific adenine glycosylase (EC 3.2.2.-)
- PFAM: NUDIX (CL0261) NUDIX_4; NUDIX domain (PF14815; HMM-score: 78.6)and 5 moreHHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 55.7)HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 28.1)no clan defined EndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 16.9)NUDIX (CL0261) NUDIX; NUDIX domain (PF00293; HMM-score: 15.4)no clan defined NDUFB10; NADH-ubiquinone oxidoreductase subunit 10 (PF10249; HMM-score: 12.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002987
- TAT(Tat/SPI): 0.000176
- LIPO(Sec/SPII): 0.000808
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQVKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEGLVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQSTRKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKLPVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKIGHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPVPMSKIYQFING
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1]
- quantitative data / protein copy number per cell: 35 [2]
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e)