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Revision as of 23:15, 10 March 2016 by AureoSysAdmin (talk | contribs) (Text replacement - "gene Genbank" to "gene RefSeq")
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NCBI date : _

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1397 [new locus tag: SACOL_RS07110 ]
  • pan locus tag?: SAUPAN003750000
  • symbol: msrA
  • pan gene symbol?: msrA2
  • synonym:
  • product: methionine sulfoxide reductase A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1397 [new locus tag: SACOL_RS07110 ]
  • symbol: msrA
  • product: methionine sulfoxide reductase A
  • replicon: chromosome
  • strand: -
  • coordinates: 1407982..1408491
  • length: 510
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3237919 NCBI
  • RefSeq: YP_186249 NCBI

⊟Phenotype[edit | edit source]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATATTAATACAGCTTATTTTGCCGGAGGTTGCTTTTGGTGTATGACGAAACCATTT
    GACACCTTTGACGGCATAGAAAAAGTAACTTCTGGATATATGGGCGGACATATTGAAAAT
    CCTACTTACGAACAAGTAAAATCAGGTACGAGTGGTCATTTAGAAACTGTTGAAATTCAA
    TATGATGTTGCATTATTCTCATACAATAAGTTATTAGAAATATTTTTCTCAGTCATTGAC
    CCATTAGATACAGGTGGTCAATATCAAGACCGTGGTCCTCAATATCAAACAGCTATTTTC
    TACACTAATGATCATCAAAAAGAACTCGCTGAGACTTATATCGAGCAGCTTAAAAATACG
    ATTAATGCTGATAAGGCAATTGCAACAAAAATATTACCAGCGTCACAATTTTACAAAGCC
    GAAGACTATCACCAAGATTTTTATAAGAAAAATCCAGAGCGCTATGCAGAAGAACAAAAA
    ATACGCCAAGAATACAAAAATAAGCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    510

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1397 [new locus tag: SACOL_RS07110 ]
  • symbol: MsrA
  • description: methionine sulfoxide reductase A
  • length: 169
  • theoretical pI: 4.86349
  • theoretical MW: 19610.7
  • GRAVY: -0.739645

⊟Function[edit | edit source]

  • reaction:
    EC 1.8.4.11?  ExPASy
    Peptide-methionine (S)-S-oxide reductase Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin
  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 188.7)
    Genetic information processing Protein fate Protein modification and repair peptide-methionine (S)-S-oxide reductase (TIGR00401; EC 1.8.4.11; HMM-score: 188.7)
  • TheSEED  :
    • Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)
    Protein Metabolism Protein processing and modification Peptide methionine sulfoxide reductase  Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)
  • PFAM:
    no clan defined PMSR; Peptide methionine sulfoxide reductase (PF01625; HMM-score: 198.7)
    and 2 more
    RCDG1; Renal cancer differentiation gene 1 protein (PF15725; HMM-score: 14.4)
    DUF4298; Domain of unknown function (DUF4298) (PF14131; HMM-score: 13)

⊟Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • interaction partners:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.047787
    • TAT(Tat/SPI): 0.000356
    • LIPO(Sec/SPII): 0.012765
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 57650365 NCBI
  • UniProt: Q5HG58 UniProt
  • protein Genbank : _
  • RefSeq: YP_186249 NCBI

⊟Protein sequence[edit | edit source]

  • MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYEQVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYTNDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQKIRQEYKNKQ

⊟Peptides[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • sigma factors : _
  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Stability[edit | edit source]

  • half-life: 95.63 h [1]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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