NCBI date : _
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0821 [new locus tag: SACOL_RS04220 ]
- pan locus tag?: SAUPAN002670000
- symbol: SACOL0821
- pan gene symbol?: —
- synonym:
- product: HD domain-containing protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0821 [new locus tag: SACOL_RS04220 ]
- symbol: SACOL0821
- product: HD domain-containing protein
- replicon: chromosome
- strand: +
- coordinates: 844394..845044
- length: 651
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237571 NCBI
- gene Genbank : _
⊟Phenotype[edit | edit source]
- Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601ATGGGTGTACATCAATATTTTAAAAGATTATCAGATATGGAAAGACTTATAAGATTACCT
GGAAAATTTAAATATTTTGAACACAATGTTGCAGCACACTCCTTTAAAGTAACTAAAATT
GCACAATATCTAGCAACAGTTGAAGAATATCATGGACGAAAGATTAATTGGAAAAGCTTA
TATGAAAAAGCATTAAATCATGATTTCGCCGAAGTGTTTACTGGTGATATAAAAACACCT
GTTAAATATGCGAGTAGTGAATTAAAAAAATTATTTTCGCAAGTTGAAGAAGAAATGGTA
GAGACCTTTATTGAAGAAGAAATTCCATTACAATATAGAGATGTTTATAAGCAACGACTG
CAAGAAGGTAAAGATGATTCATTAGAAGGCCAAATACTTTCAGTTGCTGATAAAATTGAT
TTGCTTTATGAAACATTTGGAGAAATACAAAAACGTAATCCCGAAGAATTATTTTTCGAA
ATTTATGAAATGAGTCTAGAAACAATTATTCAATTTGACCATTTAGCATCTGTACAAGAT
TTTATTAATAATATCATTCCAGAAATGTTGACTGAAAACTTTATACCTAGAACAGAATTA
AGAGAAACAACCATGAACATTTTAAATAAAAGAAAAGAGGAAAATGAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0821 [new locus tag: SACOL_RS04220 ]
- symbol: SACOL0821
- description: HD domain-containing protein
- length: 216
- theoretical pI: 4.80408
- theoretical MW: 25704
- GRAVY: -0.559722
⊟Function[edit | edit source]
- TIGRFAM: Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 16)
- TheSEED :
- Nucleotidase YfbR, HD superfamily
- PFAM: HD_PDEase (CL0237) HD_2; HD containing hydrolase-like enzyme (PF12917; HMM-score: 280)and 2 moreHD_3; HD domain (PF13023; HMM-score: 34.1)HD; HD domain (PF01966; HMM-score: 19.4)
⊟Structure, modifications & interactions[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- interaction partners:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002732
- TAT(Tat/SPI): 0.00025
- LIPO(Sec/SPII): 0.000669
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYLATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE
⊟Peptides[edit | edit source]
- experimentally validated: PeptideAtlas
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- sigma factors : _
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Stability[edit | edit source]
- half-life: 44.84 h [1]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)