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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0697 [new locus tag: SACOL_RS03585 ]
  • pan locus tag?: SAUPAN002517000
  • symbol: tagX
  • pan gene symbol?: tagX
  • synonym:
  • product: teichoic acid biosynthesis protein X

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0697 [new locus tag: SACOL_RS03585 ]
  • symbol: tagX
  • product: teichoic acid biosynthesis protein X
  • replicon: chromosome
  • strand: +
  • coordinates: 721291..722352
  • length: 1062
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGGTTTACGATAATCATACCTACATGTAATAATGAGGCAACAATTCGACAATTGTTA
    ATATCTATTGAGAGTAAAGAACACTATAGAATCCTTTGTATTGATGGTGGTTCTACTGAT
    CAAACAATTCCTATGATTGAACGGTTACAAAGAGAACTCAAGCATATTTCATTAATACAA
    TTACAAAATGCTTCGATAGCTACGTGTATTAATAAAGGTTTGATGGATATCAAAATGACA
    GATCCACATGATAGTGACGCATTTATGGTCATAAAACCAACATCAATCGTATTGCCAGGT
    AAATTAGATAGGTTAACTGCTGCTTTCAAAAATAATGATAATATTGATATGGTAATAGGG
    CAGCGAGCTTACAATTACCATGGTGAATGGAAATTGAAAAGTGCTGATGAGTTTATTAAA
    GACAATCGAATCGTTACATTAACGGAACAACCAGATTTGTTATCAATGATGTCTTTTGAC
    GGAAAGTTATTCAGTGCTAAATTTGCTGAATTACAGTGTGACGAAACTTTAGCTAACACA
    TACAATCACGCAATACTTGTCAAGGCGATGCAAAAAGCTACGGATATACATTTAGTTTCA
    CAGATGATTGTCGGAGATAACGATATAGATACACATGCTACAAGTAACGATGAAGATTTT
    AATAGATATATCACAGAAATTATGAAAATAAGACAACGAGTCATGGAAATGTTACTATTA
    CCTGAACAAAGGCTATTATATAGTGATATGGTTGATCGTATTTTATTCAATAATTCATTA
    AAATATTATATGAACGAACACCCAGCAGTAACGCACACGACAATTCAACTCGTAAAAGAC
    TATATTATGTCTATGCAGCATTCTGATTATGTATCGCAAAACATGTTTGACATTATAAAT
    ACAGTTGAATTTATTGGTGAGAATTGGGATAGAGAAATATACGAATTGTGGCGACAAACA
    TTAATTCAAGTGGGCATTAATAGGCCGACTTATAAAAAATTCTTGATACAACTTAAAGGG
    AGAAAGTTTGCACATCGAACAAAATCAATGTTAAAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0697 [new locus tag: SACOL_RS03585 ]
  • symbol: TagX
  • description: teichoic acid biosynthesis protein X
  • length: 353
  • theoretical pI: 6.91091
  • theoretical MW: 41138.4
  • GRAVY: -0.298017

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.-.-?  ExPASy
  • TIGRFAM:
    poly-beta-1,6 N-acetyl-D-glucosamine synthase (TIGR03937; EC 2.4.1.-; HMM-score: 28)
    Unknown function Enzymes of unknown specificity transferase 2, rSAM/selenodomain-associated (TIGR04283; EC 2.-.-.-; HMM-score: 27.8)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 26.5)
    and 1 more
    mycofactocin system glycosyltransferase (TIGR03965; HMM-score: 15.8)
  • TheSEED  :
    • Teichoic acid biosynthesis protein X
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Teichoic acid biosynthesis protein X
  • PFAM:
    GT-A (CL0110) Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 41.1)
    and 3 more
    Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 21.8)
    Glyco_tranf_2_2; Glycosyltransferase like family 2 (PF10111; HMM-score: 16.8)
    no clan defined Thg1; tRNAHis guanylyltransferase (PF04446; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016492
    • TAT(Tat/SPI): 0.000131
    • LIPO(Sec/SPII): 0.003167
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIPMIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKLDRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFDGKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDEDFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQLVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKFLIQLKGRKFAHRTKSMLKR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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