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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1587 [new locus tag: SA_RS08945 ]
  • pan locus tag?: SAUPAN004456000
  • symbol: ribA
  • pan gene symbol?: ribA
  • synonym:
  • product: riboflavin biosynthesis protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1587 [new locus tag: SA_RS08945 ]
  • symbol: ribA
  • product: riboflavin biosynthesis protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1825444..1826625
  • length: 1182
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAAACAATTATT
    GTAGTAGATGATGAGAATCGTGAAAATGAAGGTGATTTAGTAGCGGTTACTGAATGGATG
    AACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGGGATTAATATGCGCACCAGTG
    TCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
    TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGTGCT
    TATGAACGTACATTGACTGCCAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTT
    AATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATAAAGGCGTATTAGCTAGAAAT
    GGACACACAGAAGCGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
    ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAATTTT
    AAAGAAAAACATCAATTAAAGATGATTACGATTGATGATTTAATTGAATATCGTAAAAAA
    TTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGCCTACAGATTTCGGAACATTT
    GATATGTATGGTTTTAAAGCGACATACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
    GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTC
    CATAGTCAACGTTGTGATTGTGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAA
    CATGGTGGCATGATTATTTATCTACCTCAAGAAGGTCGTGGCATAGGATTGTTAAACAAA
    TTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
    GGTTTTGATGAAGATTTACGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAAC
    ATCGAACATATCAATTTATTAAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATAT
    GGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAGAAACGGTACATAATCATGAT
    TATATGGTAACGAAAAAAATAAAAATGGGTCATTTAATATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1587 [new locus tag: SA_RS08945 ]
  • symbol: RibA
  • description: riboflavin biosynthesis protein
  • length: 393
  • theoretical pI: 4.87996
  • theoretical MW: 44148
  • GRAVY: -0.303817

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.4.25?  ExPASy
    GTP cyclohydrolase II GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
    EC 4.1.99.12?  ExPASy
    3,4-dihydroxy-2-butanone-4-phosphate synthase D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD 3,4-dihydroxy-2-butanone-4-phosphate synthase (TIGR00506; EC 4.1.99.12; HMM-score: 247.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD GTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 224.8)
  • TheSEED  :
    • 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12)
    • GTP cyclohydrolase II (EC 3.5.4.25)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  GTP cyclohydrolase II (EC 3.5.4.25)
    and 2 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  GTP cyclohydrolase II (EC 3.5.4.25)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD riboflavin to FAD  3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12)
  • PFAM:
    no clan defined DHBP_synthase; 3,4-dihydroxy-2-butanone 4-phosphate synthase (PF00926; HMM-score: 266)
    and 1 more
    GTP_cyclohydro2; GTP cyclohydrolase II (PF00925; HMM-score: 209.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+, Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002287
    • TAT(Tat/SPI): 0.000212
    • LIPO(Sec/SPII): 0.000578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQNFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMVTKKIKMGHLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FMN-box (transcription termination) regulon
    FMN-box(RNA)important in Riboflavin biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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