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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0363 [new locus tag: SA_RS02080 ]
  • pan locus tag?: SAUPAN001978000
  • symbol: SA0363
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0363 [new locus tag: SA_RS02080 ]
  • symbol: SA0363
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 419940..420566
  • length: 627
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAAAGAGATTACTACTAAGTACATTTTTAGCATCTGCATTAATTCTTACAGGTTGC
    GCGTCCGATCAATCTGATAACGAAGATCATCACACAAGTACTGGCATCCATGCACCTAAA
    AGCGCTAAAAAATTAGAAACAAAAGATATCTTTAACTCAGATAAGAAAAATAGTGATATT
    AGTGATGCAGAGATGAAGCAAGCTATAGAAAAGTATTTGTCTGTAAATAGCGATATACTT
    GATAACAAATATATTATGCAACATAAACTAGATAAACAAATTGACAGTCAAACAAAAGTG
    ACTGAAAAACAAGCAGAAACACTTAGCCACTTATCTAACTTAGCTGTTAAAAACGATTTG
    CATTTCAAGAAATTTGTCACTGAAAACAATATTCCTAAAGAATATAAGAAACCCGTTGAA
    TTAATGATGAATTACTTCAAAGCTTTAAATAGTACAATCGCCAATGTTGATGAAGATATT
    GAAAAGCTTAGCTATCAACCGCAAAATAAAATCAATGTAGTCGACGTGCCAACAAAATAT
    GCCGGTGATGTTAATAAAAAGCAACAAGATAAAATTAAAGATTTCTTAAAATCTAAAGGT
    ATCAAGAGTGATGTTATCGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0363 [new locus tag: SA_RS02080 ]
  • symbol: SA0363
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 9.1737
  • theoretical MW: 23633.8
  • GRAVY: -0.747115

Function[edit | edit source]

  • TIGRFAM:
    extracellular lipase, Pla-1/cef family (TIGR03502; EC 3.1.-.-; HMM-score: 20.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking outer membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 19.3)
    and 1 more
    Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 12)
  • TheSEED  :
    • Uncharacterized protein SERP0057
  • PFAM:
    no clan defined Pilus_CpaD; Pilus biogenesis CpaD protein (pilus_cpaD) (PF09476; HMM-score: 14.5)
    DUF1748; Fungal protein of unknown function (DUF1748) (PF08520; HMM-score: 13.7)
    and 1 more
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 11.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LILTGCA
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000497
    • TAT(Tat/SPI): 0.000069
    • LIPO(Sec/SPII): 0.999225
    • Cleavage Site: CS pos: 19-20. LTG-CA. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKKLETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTEKQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDIEKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]