PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI date : _

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS03650 [old locus tag: NWMN_0642 ]
• pan locus tag^?: SAUPAN002556000
• symbol: NWMN_RS03650
• pan gene symbol^?: —
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS03650 [old locus tag: NWMN_0642 ]
• symbol: NWMN_RS03650
• product: MFS transporter
• replicon: chromosome
• strand: +
• coordinates: 725671..726891
• length: 1221
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• gene Genbank : _

⊟Phenotype

• Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGGATATGTTTGCAGCGTTATTACAAATAAAGAATTATAAACTCTTTGTTGCTAATATG
  TTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAA
  GATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAG
  TTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATT
  ATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGAT
  ACAATCTGGTTATTCATATTACTATATGCGATTTTCCAAGGATTATTTGCACCAGCAATG
  CCCCAACTTTACGCATCTGCTAGAGAATCTATCAATGTTTCAAGCTCTAAAGATAGAGCT
  CAATTTGCCAACACAGTATTACGTTCAATGTTCTCATTGGGCTTTTTATTTGGTCCATTT
  ATTGGTGCCCAATTAATCGGATTAAAAGGCTATGCTGGATTGTTTGGTGGAACAATAAGT
  ATCATTTTATTTACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACAC
  CCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACG
  CTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATG
  AATATGCCTTTATTTGTTACTGATTATTTAAAAGAAAATGAACAACATGTCGGTTATTTA
  GCTAGTTTATGTGCTGGTTTAGAAGTGCCATTTATGATCATTCTTGGCGTTTTATCATCT
  AGATTACAGACTCGAACATTGTTGATTTATGGAGCGATTTTTGGTGGTTTATTCTACTTC
  AGCATTGGGGTATTTAAAAACTTCTATATGATGTTAGCAGGACAGGTGTTTTTAGCTATT
  TTCTTAGCGGTTCTTTTAGGAATTGGTATTAGTTATTTCCAAGATATCTTACCAGATTTT
  CCAGGATACGCTTCAACACTATTTTCTAATGCAATGGTTATTGGACAGTTAGGCGGTAAC
  CTATTAGGTGGTGCTATGAGTCACTGGGTAGGTTTGGAAAATGTATTTTTTGTATCAGCA
  GCATCAATCATGTTAGGTATGATACTTATATTCTTTACTAAAAATCAAAAAATTACAAAA
  GAGGATGTGATATCAACATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1221
  

⊟Protein

⊟General

• locus tag: NWMN_RS03650 [old locus tag: NWMN_0642 ]
• symbol: NWMN_RS03650
• description: MFS transporter
• length: 406
• theoretical pI: 9.39681
• theoretical MW: 45130.5
• GRAVY: 0.813054

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 161.2)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 44.8)
  multidrug resistance protein (TIGR00880; HMM-score: 41.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 17.1)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 120.4)
  and 3 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.5)
  ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 17.5)
  no clan defined Ilm1; Increased loss of mitochondrial DNA protein 1 (PF10311; HMM-score: 12.1)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• interaction partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016845
  • TAT(Tat/SPI): 0.00127
  • LIPO(Sec/SPII): 0.006278
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446289209 NCBI
• UniProt: see NWMN_0642
• RefSeq: WP_000367064 NCBI

⊟Protein sequence

• MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGMTTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKITKEDVIST

⊟Peptides

• experimentally validated: data available for COL, NCTC8325

⊟Expression & Regulation

⊟Operon

⊟Regulation

• sigma factors : _
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications