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m (Text replacement - "gene Genbank" to "gene RefSeq")
 
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__TOC__
<protect>
<protect>
<aureodatabase>NCBI date</aureodatabase>
<aureodatabase>annotation</aureodatabase>


=Summary=
=Summary=


* <aureodatabase>organism</aureodatabase>
*<aureodatabase>organism</aureodatabase>
* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>pan locus</aureodatabase>
*<aureodatabase>pan locus</aureodatabase>
* <aureodatabase>gene symbol</aureodatabase>
*<aureodatabase>gene symbol</aureodatabase>
* <aureodatabase>pan gene symbol</aureodatabase>
*<aureodatabase>pan gene symbol</aureodatabase>
* <aureodatabase>gene synonyms</aureodatabase>
*<aureodatabase>gene synonyms</aureodatabase>
* <aureodatabase>product</aureodatabase>
*<aureodatabase>product</aureodatabase>
</protect>
</protect>


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==General==
==General==


* <aureodatabase>gene type</aureodatabase>
*<aureodatabase>gene type</aureodatabase>
* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>gene symbol</aureodatabase>
*<aureodatabase>gene symbol</aureodatabase>
* <aureodatabase>product</aureodatabase>
*<aureodatabase>product</aureodatabase>
* <aureodatabase>gene replicon</aureodatabase>
*<aureodatabase>gene replicon</aureodatabase>
* <aureodatabase>strand</aureodatabase>
*<aureodatabase>strand</aureodatabase>
* <aureodatabase>gene coordinates</aureodatabase>
*<aureodatabase>gene coordinates</aureodatabase>
* <aureodatabase>gene length</aureodatabase>
*<aureodatabase>gene length</aureodatabase>
* <aureodatabase>essential</aureodatabase>
*<aureodatabase>essential</aureodatabase>
*<aureodatabase>gene comment</aureodatabase>
</protect>
</protect>


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==Accession numbers==
==Accession numbers==


* <aureodatabase>gene GI</aureodatabase>
*<aureodatabase>gene location</aureodatabase>
* <aureodatabase>gene Genbank</aureodatabase>
*<aureodatabase>gene BioCyc</aureodatabase>
*<aureodatabase>gene MicrobesOnline</aureodatabase>
</protect>
</protect>
   
   
<protect>  
<protect>
==Phenotype==
==Phenotype==
</protect>
</protect>
* Share your knowledge and add information here. [<span class="plainlinks">[http://www.protecs.uni-greifswald.de/aureowiki/index.php?title={{PAGENAMEE}}&action=edit&section=6 edit]</span>]
Share your knowledge and add information here. [<span class="plainlinks">[//aureowiki.med.uni-greifswald.de/index.php?title={{PAGENAMEE}}&veaction=edit&section=6 edit]</span>]


<protect>
<protect>
==DNA sequence==
==DNA sequence==


* <aureodatabase>gene sequence</aureodatabase>
*<aureodatabase>gene sequence</aureodatabase>
</protect>
</protect>


<protect>
<protect>
<aureodatabase>RNA regulated operons</aureodatabase>
</protect>


<protect>
=Protein=
=Protein=
<aureodatabase>protein 3D view</aureodatabase>
<aureodatabase>protein 3D view</aureodatabase>
==General==
==General==


* <aureodatabase>locus</aureodatabase>
*<aureodatabase>locus</aureodatabase>
* <aureodatabase>protein symbol</aureodatabase>
*<aureodatabase>protein symbol</aureodatabase>
* <aureodatabase>protein description</aureodatabase>
*<aureodatabase>protein description</aureodatabase>
* <aureodatabase>protein length</aureodatabase>
*<aureodatabase>protein length</aureodatabase>
* <aureodatabase>theoretical pI</aureodatabase>
*<aureodatabase>theoretical pI</aureodatabase>
* <aureodatabase>theoretical MW</aureodatabase>
*<aureodatabase>theoretical MW</aureodatabase>
* <aureodatabase>GRAVY</aureodatabase>
*<aureodatabase>GRAVY</aureodatabase>
</protect>
</protect>


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==Function==
==Function==


* <aureodatabase>protein reaction</aureodatabase>
*<aureodatabase>protein reaction</aureodatabase>
* <aureodatabase>protein TIGRFAM</aureodatabase>
*<aureodatabase>protein TIGRFAM</aureodatabase>
* <aureodatabase>protein TheSeed</aureodatabase>
*<aureodatabase>protein TheSeed</aureodatabase>
* <aureodatabase>protein PFAM</aureodatabase>
*<aureodatabase>protein PFAM</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Structure, modifications & interactions==
==Structure, modifications & cofactors==


* <aureodatabase>protein domains</aureodatabase>
*<aureodatabase>protein domains</aureodatabase>
* <aureodatabase>protein modifications</aureodatabase>
*<aureodatabase>protein modifications</aureodatabase>
* <aureodatabase>protein cofactors</aureodatabase>
*<aureodatabase>protein cofactors</aureodatabase>
* <aureodatabase>protein effectors</aureodatabase>
*<aureodatabase>protein effectors</aureodatabase>
* <aureodatabase>protein partners</aureodatabase>
*<aureodatabase>protein regulated operons</aureodatabase>
</protect>
</protect>


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==Localization==
==Localization==


* <aureodatabase>protein Psortb</aureodatabase>
*<aureodatabase>protein Psortb</aureodatabase>
* <aureodatabase>protein LocateP</aureodatabase>
*<aureodatabase>protein LocateP</aureodatabase>
* <aureodatabase>protein SignalP</aureodatabase>
*<aureodatabase>protein SignalP</aureodatabase>
* <aureodatabase>protein TMHMM</aureodatabase>
*<aureodatabase>protein TMHMM</aureodatabase>
</protect>
</protect>


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==Accession numbers==
==Accession numbers==


* <aureodatabase>protein GI</aureodatabase>
*<aureodatabase>protein GI</aureodatabase>
* <aureodatabase>protein UniProt</aureodatabase>
*<aureodatabase>protein RefSeq</aureodatabase>
* <aureodatabase>protein RefSeq</aureodatabase>
*<aureodatabase>protein UniProt</aureodatabase>
</protect>
</protect>


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==Protein sequence==
==Protein sequence==


* <aureodatabase>protein sequence</aureodatabase>
*<aureodatabase>protein sequence</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Peptides==
==Experimental data==


* <aureodatabase>protein validated peptides</aureodatabase>
*<aureodatabase>protein validated peptides</aureodatabase>
*<aureodatabase>protein validated localization</aureodatabase>
*<aureodatabase>protein validated quantitative data</aureodatabase>
*<aureodatabase>protein partners</aureodatabase>
</protect>
</protect>


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==Operon==
==Operon==


* <aureodatabase>operons</aureodatabase>
*<aureodatabase>operons</aureodatabase>
</protect>
</protect>


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==Regulation==
==Regulation==


* <aureodatabase>sigma factors</aureodatabase>
*<aureodatabase>regulators</aureodatabase>
* <aureodatabase>regulators</aureodatabase>
</protect>
</protect>


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==Transcription pattern==
==Transcription pattern==


* <aureodatabase>expression browser</aureodatabase>
*<aureodatabase>expression browser</aureodatabase>
</protect>
</protect>


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==Protein synthesis (provided by Aureolib)==
==Protein synthesis (provided by Aureolib)==


* <aureodatabase>protein synthesis Aureolib</aureodatabase>
*<aureodatabase>protein synthesis Aureolib</aureodatabase>
</protect>
</protect>


<protect>
<protect>
==Stability==
==Protein stability==


* <aureodatabase>protein half-life</aureodatabase>
*<aureodatabase>protein half-life</aureodatabase>
</protect>
</protect>


Latest revision as of 00:30, 11 March 2016

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • pan locus tag?: SAUPAN002473000
  • symbol: NWMN_RS03315
  • pan gene symbol?: argS
  • synonym:
  • product: arginine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • product: arginine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 659531..661192
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (659531..661192) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0579

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATATTATTGATCAAGTGAAACAAACATTAGTAGAAGAAATTGCAGCAAGTATTAAC
    AAAGCAGGATTAGCAGATGAGATTCCTGATATTAAAATTGAAGTTCCTAAAGATACAAAA
    AATGGAGATTATGCTACTAATATTGCGATGGTACTGACTAAGATTGCAAAGCGTAATCCT
    CGTGAAATTGCTCAAGCGATTGTTGATAACTTAGATACTGAAAAAGCACATGTAAAACAA
    ATTGACATTGCTGGTCCAGGATTCATTAATTTTTACTTAGATAATCAGTATTTAACAGCA
    ATTATTCCTGAAGCAATTGAAAAAGGTGATCAATTTGGACATGTAAATGAATCAAAAGGT
    CAAAATGTATTGCTTGAGTATGTTTCAGCTAACCCTACAGGAGATTTACATATTGGTCAT
    GCTAGAAATGCAGCAGTTGGTGATGCTTTAGCTAATATTTTAACTGCAGCTGGCTATAAT
    GTAACACGTGAATATTATATTAATGATGCTGGTAATCAAATTACTAACTTAGCGCGTTCG
    ATTGAAACACGTTTCTTTGAAGCTTTAGGTGACAATAGTTATTCAATGCCAGAAGATGGC
    TATAATGGAAAAGATATTATTGAAATAGGTAAAGATTTAGCAGAGAAACACCCTGAAATT
    AAAGATTATTCTGAAGAAGCACGTTTGAAAGAATTTAGAAAATTAGGCGTAGAATACGAA
    ATGGCTAAATTGAAAAATGATTTAGCAGAGTTCAATACGCATTTTGATAATTGGTTTAGT
    GAAACATCTTTATATGAAAAAGGAGAAATTCTTGAAGTTTTAGCAAAAATGAAAGAATTA
    GGTTATACGTATGAAGCTGATGGCGCTACATGGTTACGTACAACTGATTTTAAAGACGAC
    AAAGACAGAGTATTAATTAAAAATGACGGTACATATACGTATTTCTTACCAGATATTGCG
    TACCACTTCGATAAAGTAAAACGTGGTAATGACATTTTAATCGATTTATTTGGTGCTGAT
    CATCATGGTTATATTAATCGTTTGAAAGCATCTCTTGAAACGTTTGGTGTAGATAGTAAT
    CGTTTAGAAATTCAAATCATGCAAATGGTTCGTTTAATGGAAAATGGTAAAGAAGTGAAG
    ATGAGTAAACGTACTGGTAATGCGATTACATTAAGAGAAATTATGGACGAAGTTGGCGTT
    GACGCTGCACGTTATTTCTTAACTATGCGTAGTCCTGATAGTCACTTTGATTTTGATATG
    GAATTAGCGAAAGAGCAATCTCAAGACAATCCAGTTTACTATGCTCAATATGCACATGCG
    CGTATTTGTTCAATTTTAAAACAAGCGAAAGAGCAAGGTATTGAAGTGACTGCTGCGAAT
    GATTTTACAACGATTACTAATGAAAAAGCGATTGAATTGTTGAAAAAAGTAGCTGATTTC
    GAACCTACAATTGAAAGTGCTGCTGAGCATAGATCGGCACATAGAATTACTAATTATATT
    CAAGATTTAGCTTCTCATTTCCATAAATTCTATAATGCTGAAAAAGTGTTAACAGATGAT
    ATTGAAAAAACAAAAGCACATGTTGCTATGATTGAAGCGGTCAGAATTACATTGAAAAAT
    GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • description: arginine--tRNA ligase
  • length: 553
  • theoretical pI: 4.86205
  • theoretical MW: 62380.9
  • GRAVY: -0.425316

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.19?  ExPASy
    Arginine--tRNA ligase ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 511.7)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 42.6)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 41.6)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 37.1)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 33.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 31.4)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 22.7)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 20.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    DALR (CL0258) DALR_1; DALR anticodon binding domain (PF05746; HMM-score: 123.1)
    and 9 more
    no clan defined Arg_tRNA_synt_N; Arginyl tRNA synthetase N terminal domain (PF03485; HMM-score: 90.2)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 89.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 38.3)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 33.9)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 31.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 28)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 16.2)
    tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 15.7)
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006584
    • TAT(Tat/SPI): 0.000488
    • LIPO(Sec/SPII): 0.001017
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDYATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAIIPEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYNVTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHPEIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAKMKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILIDLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLREIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAKEQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFHKFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS07455dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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