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m (Text replacement - "gene Genbank" to "gene RefSeq") |
m (Text replacement - "* <aureodatabase>protein Genbank</aureodatabase> " to "") |
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__TOC__ | |||
<protect> | <protect> | ||
<aureodatabase> | <aureodatabase>annotation</aureodatabase> | ||
=Summary= | =Summary= | ||
* <aureodatabase>organism</aureodatabase> | *<aureodatabase>organism</aureodatabase> | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>pan locus</aureodatabase> | *<aureodatabase>pan locus</aureodatabase> | ||
* <aureodatabase>gene symbol</aureodatabase> | *<aureodatabase>gene symbol</aureodatabase> | ||
* <aureodatabase>pan gene symbol</aureodatabase> | *<aureodatabase>pan gene symbol</aureodatabase> | ||
* <aureodatabase>gene synonyms</aureodatabase> | *<aureodatabase>gene synonyms</aureodatabase> | ||
* <aureodatabase>product</aureodatabase> | *<aureodatabase>product</aureodatabase> | ||
</protect> | </protect> | ||
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==General== | ==General== | ||
* <aureodatabase>gene type</aureodatabase> | *<aureodatabase>gene type</aureodatabase> | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>gene symbol</aureodatabase> | *<aureodatabase>gene symbol</aureodatabase> | ||
* <aureodatabase>product</aureodatabase> | *<aureodatabase>product</aureodatabase> | ||
* <aureodatabase>gene replicon</aureodatabase> | *<aureodatabase>gene replicon</aureodatabase> | ||
* <aureodatabase>strand</aureodatabase> | *<aureodatabase>strand</aureodatabase> | ||
* <aureodatabase>gene coordinates</aureodatabase> | *<aureodatabase>gene coordinates</aureodatabase> | ||
* <aureodatabase>gene length</aureodatabase> | *<aureodatabase>gene length</aureodatabase> | ||
* <aureodatabase>essential</aureodatabase> | *<aureodatabase>essential</aureodatabase> | ||
*<aureodatabase>gene comment</aureodatabase> | |||
</protect> | </protect> | ||
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==Accession numbers== | ==Accession numbers== | ||
* <aureodatabase>gene GI</aureodatabase> | *<aureodatabase>gene GI</aureodatabase> | ||
* <aureodatabase>gene RefSeq</aureodatabase> | *<aureodatabase>gene RefSeq</aureodatabase> | ||
*<aureodatabase>gene BioCyc</aureodatabase> | |||
*<aureodatabase>gene MicrobesOnline</aureodatabase> | |||
</protect> | </protect> | ||
<protect> | <protect> | ||
==Phenotype== | ==Phenotype== | ||
</protect> | </protect> | ||
Share your knowledge and add information here. [<span class="plainlinks">[//aureowiki.med.uni-greifswald.de/index.php?title={{PAGENAMEE}}&veaction=edit§ion=6 edit]</span>] | |||
<protect> | <protect> | ||
==DNA sequence== | ==DNA sequence== | ||
* <aureodatabase>gene sequence</aureodatabase> | *<aureodatabase>gene sequence</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
<aureodatabase>RNA regulated operons</aureodatabase> | |||
</protect> | |||
<protect> | |||
=Protein= | =Protein= | ||
<aureodatabase>protein 3D view</aureodatabase> | <aureodatabase>protein 3D view</aureodatabase> | ||
==General== | ==General== | ||
* <aureodatabase>locus</aureodatabase> | *<aureodatabase>locus</aureodatabase> | ||
* <aureodatabase>protein symbol</aureodatabase> | *<aureodatabase>protein symbol</aureodatabase> | ||
* <aureodatabase>protein description</aureodatabase> | *<aureodatabase>protein description</aureodatabase> | ||
* <aureodatabase>protein length</aureodatabase> | *<aureodatabase>protein length</aureodatabase> | ||
* <aureodatabase>theoretical pI</aureodatabase> | *<aureodatabase>theoretical pI</aureodatabase> | ||
* <aureodatabase>theoretical MW</aureodatabase> | *<aureodatabase>theoretical MW</aureodatabase> | ||
* <aureodatabase>GRAVY</aureodatabase> | *<aureodatabase>GRAVY</aureodatabase> | ||
</protect> | </protect> | ||
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==Function== | ==Function== | ||
* <aureodatabase>protein reaction</aureodatabase> | *<aureodatabase>protein reaction</aureodatabase> | ||
* <aureodatabase>protein TIGRFAM</aureodatabase> | *<aureodatabase>protein TIGRFAM</aureodatabase> | ||
* <aureodatabase>protein TheSeed</aureodatabase> | *<aureodatabase>protein TheSeed</aureodatabase> | ||
* <aureodatabase>protein PFAM</aureodatabase> | *<aureodatabase>protein PFAM</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
==Structure, modifications & | ==Structure, modifications & cofactors== | ||
* <aureodatabase>protein domains</aureodatabase> | *<aureodatabase>protein domains</aureodatabase> | ||
* <aureodatabase>protein modifications</aureodatabase> | *<aureodatabase>protein modifications</aureodatabase> | ||
* <aureodatabase>protein cofactors</aureodatabase> | *<aureodatabase>protein cofactors</aureodatabase> | ||
* <aureodatabase>protein effectors</aureodatabase> | *<aureodatabase>protein effectors</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein regulated operons</aureodatabase> | ||
</protect> | </protect> | ||
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==Localization== | ==Localization== | ||
* <aureodatabase>protein Psortb</aureodatabase> | *<aureodatabase>protein Psortb</aureodatabase> | ||
* <aureodatabase>protein LocateP</aureodatabase> | *<aureodatabase>protein LocateP</aureodatabase> | ||
* <aureodatabase>protein SignalP</aureodatabase> | *<aureodatabase>protein SignalP</aureodatabase> | ||
* <aureodatabase>protein TMHMM</aureodatabase> | *<aureodatabase>protein TMHMM</aureodatabase> | ||
</protect> | </protect> | ||
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==Accession numbers== | ==Accession numbers== | ||
* <aureodatabase>protein GI</aureodatabase> | *<aureodatabase>protein GI</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein RefSeq</aureodatabase> | ||
* <aureodatabase>protein | *<aureodatabase>protein UniProt</aureodatabase> | ||
</protect> | </protect> | ||
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==Protein sequence== | ==Protein sequence== | ||
* <aureodatabase>protein sequence</aureodatabase> | *<aureodatabase>protein sequence</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
== | ==Experimental data== | ||
* <aureodatabase>protein validated peptides</aureodatabase> | *<aureodatabase>protein validated peptides</aureodatabase> | ||
*<aureodatabase>protein validated localization</aureodatabase> | |||
*<aureodatabase>protein validated quantitative data</aureodatabase> | |||
*<aureodatabase>protein partners</aureodatabase> | |||
</protect> | </protect> | ||
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==Operon== | ==Operon== | ||
* <aureodatabase>operons</aureodatabase> | *<aureodatabase>operons</aureodatabase> | ||
</protect> | </protect> | ||
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==Regulation== | ==Regulation== | ||
*<aureodatabase>regulators</aureodatabase> | |||
* <aureodatabase>regulators</aureodatabase> | |||
</protect> | </protect> | ||
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==Transcription pattern== | ==Transcription pattern== | ||
* <aureodatabase>expression browser</aureodatabase> | *<aureodatabase>expression browser</aureodatabase> | ||
</protect> | </protect> | ||
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==Protein synthesis (provided by Aureolib)== | ==Protein synthesis (provided by Aureolib)== | ||
* <aureodatabase>protein synthesis Aureolib</aureodatabase> | *<aureodatabase>protein synthesis Aureolib</aureodatabase> | ||
</protect> | </protect> | ||
<protect> | <protect> | ||
== | ==Protein stability== | ||
* <aureodatabase>protein half-life</aureodatabase> | *<aureodatabase>protein half-life</aureodatabase> | ||
</protect> | </protect> | ||
Latest revision as of 22:56, 10 March 2016
NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2253 [new locus tag: NWMN_RS12970 ]
- pan locus tag?: SAUPAN005852000
- symbol: NWMN_2253
- pan gene symbol?: emrB
- synonym:
- product: drug resistance transporter EmrB/QacA subfamily protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2253 [new locus tag: NWMN_RS12970 ]
- symbol: NWMN_2253
- product: drug resistance transporter EmrB/QacA subfamily protein
- replicon: chromosome
- strand: -
- coordinates: 2474687..2476618
- length: 1932
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331371 NCBI
- RefSeq: YP_001333287 NCBI
- BioCyc:
- MicrobesOnline: 3707855 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1921ATGACTACGACCTTCATTATTAGCTACATTATTTTAGCGCTCATTATTGTTGGGGTTATC
AATTTATTTTTAATAAGATCAAGAAAAAAAGGCAAACGCCAACAAAAGGAACAACAATTT
ACGACACGTCAATCAAATCAATCTAAATTTAAAGCTAGTGATTTAGACAAAACAACTGAT
CAATCGACACAACGTATGACGCATGAAGAGTTGCGTGTTGACAATCAAGATGATCATAGC
CAAGTTAGTCTAAATGGTTACACAAAGGGGTCTGAGAAAGATCAAGAAGCATTCACTAAT
AATAAGGATGAGGAAGCAGTTGCTGCTAAAAATCCTGAATCAGAAGAATATAAAGTGAAT
GAAAAAATAAAAAAAGAACATAAAAACTTTATTTTTGGTGAAGGTGTTTCACGCGGTAAA
ATATTAGCGGCATTATTATTCGGTATGTTTATTGCGATTTTAAACCAAACATTATTAAAT
GTTGCTTTACCAAAAATTAATACGGAATTTAATATTTCTGCGTCAACAGGGCAATGGTTG
ATGACGGGATTCATGTTAGTAAACGGTATTTTAATACCAATTACGGCGTATCTATTTAAT
AAATATTCATATCGAAAATTATTTTTAGTTGCGTTAGTACTCTTTACAATTGGTTCATTA
ATCTGTGCGATTTCAATGAATTTCCCAATCATGATGGTTGGACGTGTATTACAAGCCATT
GGTGCAGGTGTACTAATGCCATTAGGTTCAATTGTTATTATTACAATTTATCCACCTGAA
AAACGTGGTGCTGCCATGGGTACAATGGGTATCGCAATGATTTTAGCACCTGCAATTGGT
CCAACATTATCAGGTTATATTGTACAAAATTACCATTGGAATGTGATGTTTTACGGTATG
TTCATTATCGGGATTATCGCTATTTTAATTGGTTTTGTATGGTTTAAGTTATATCAATAT
ACAACAAACCCTAAAGCTGATATCCCAGGTATCATTTTTAGTACGATTGGTTTTGGTGCT
TTGTTATATGGTTTCTCAGAAGCTGGCAACAAAGGTTGGGGTTCAGTAGAGATAGAAACA
ATGTTTGCGATTGGTATTATCTTTATTATTCTATTCGTTATTAGAGAATTAAGAATGAAA
TCACCAATGTTGAATTTAGAAGTATTGAAATTCCCAACATTTACATTAACAACAATTATT
AATATGGTTGTAATGTTAAGTTTATATGGTGGTATGATTTTATTACCGATTTATTTACAA
AATTTACGCGGATTCTCAGCATTAGATTCCGGATTGTTATTATTACCTGGTTCTCTAATT
ATGGGTCTACTAGGACCATTTGCAGGTAAATTGTTAGATACTATTGGTTTAAAACCATTA
GCTATCTTTGGTATTGCTGTAATGACTTATGCAACATGGGAATTAACTAAATTAAATATG
GATACACCATATATGACAATCATGGGTATCTATGTACTTCGTTCATTTGGTATGGCATTT
ATAATGATGCCAATGGTAACTGCAGCTATTAATGCGTTACCGGGACGACTTGCCTCTCAT
GGTAATGCTTTCTTAAATACGATGCGTCAATTAGCAGGCTCTATAGGTACAGCAATCTTA
GTTACTGTAATGACAACACAAACTACACAACACTTATCAGCTTTTGGGGAAGAGTTAGAT
AAAACGAATCCTGTTGTACAAGATCATATGCGTGAATTAGCATCACAATATGGCGGACAA
GAAGGCGCAATGAAAGTGTTACTACAATTTGTAAATAAACTAGCAACGGTTGAAGGTATT
AATGATGCATTTATAGTTGCAACGATATTTAGCATCATCGCCTTAATTTTATGTTTATTT
TTACAAAGTAATAAAAAAGCAAAAGCTACAGCTCAAAAGTTAGATGCAGATAATAGTATC
AATCATGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2253 [new locus tag: NWMN_RS12970 ]
- symbol: NWMN_2253
- description: drug resistance transporter EmrB/QacA subfamily protein
- length: 643
- theoretical pI: 9.56274
- theoretical MW: 70977.5
- GRAVY: 0.398134
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.1)Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.1)and 11 moremultidrug resistance protein (TIGR00880; HMM-score: 76.8)drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 67.8)Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 64.7)Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 63.8)Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 48.1)Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 25.6)oxalate/formate antiporter (TIGR04259; HMM-score: 22.7)Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 13.9)Cellular processes Adaptations to atypical conditions phage shock protein B (TIGR02976; HMM-score: 10)synaptic vesicle protein SV2 (TIGR01299; HMM-score: 8.3)sodium-independent organic anion transporter (TIGR00805; HMM-score: 7.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 192.3)and 11 moreSugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 35.5)no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 16.6)Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 14.6)EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 14.1)ATP_synthase (CL0255) YMF19; Plant ATP synthase F0 (PF02326; HMM-score: 13.4)no clan defined ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.3)DUF2561; Protein of unknown function (DUF2561) (PF10812; HMM-score: 12.2)Shisa; Wnt and FGF inhibitory regulator (PF13908; HMM-score: 10.9)DUF1180; Protein of unknown function (DUF1180) (PF06679; HMM-score: 10.7)DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 9)MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 5.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 15
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.083803
- TAT(Tat/SPI): 0.001799
- LIPO(Sec/SPII): 0.024049
- predicted transmembrane helices (TMHMM): 15
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSINHE
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.