PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0401 [new locus tag: NWMN_RS02270 ]
• pan locus tag^?: SAUPAN002130000
• symbol: NWMN_0401
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0401 [new locus tag: NWMN_RS02270 ]
• symbol: NWMN_0401
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 450933..452441
• length: 1509
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332475 NCBI
• RefSeq: YP_001331435 NCBI
• BioCyc:
• MicrobesOnline: 3705932 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  1501
  
  ATGAGGGAAAATTTTAAGTTACGTAAAATGAAAGTCGGTTTAGTATCTGTTGCAATTACA
  ATGTTATATATTATGACAAACGGACAAGCAGAAGCATCTGAAAATCAAAACGCTTTAATC
  TCTAATATAAATGTAGACAATCAGGAAAAACAGAATAATGTAAATCAAGCTGTTCAGCCT
  CAAAATAATACTAATGAAACATCAAAAGTACCGGCTAATTTTGTCAAATTGAATGATATT
  AAACCAGGTGATACTTCTATACAAGGAACAACTTTACCAAATCAATTTATACTATTAACT
  ATTGATAAAAAAGATGTGAGCTCAGTTGAAGATTCTGACAGCAGCTTTGTTATGTCTGAT
  AAAGATGGGAATTTTAAGTATGACTTAAATGGTCGCAAAATTGTTCATAATCAAGAAATT
  GAAGTGTCTTCATCAGATCCCTATTTAGGTGACGATGAAGAAGATGAAGAAGTAGAAGAA
  ACTTCAACTGAAGAAGTTGGTGCTGAGGAAGAAAGTACAGAAGCTAAAGCTACATATACA
  ACACCGCGATATGAAAAAGCGTATGAAATACCGAAAGAACAGCTAAAAGAAAAAGATGGA
  CATCACCAAGTTTTTATCGAACCTATTACTGAAGGTTCAGGTATTATTAAAGGCCATACC
  TCTGTAAAAGGTAAAGTTGCTCTATCTATTAATAATAAATTTATTAACTTTGAGACAAAT
  GCTAATGGTGGTCCAAATAAAGAAGAAGCGAAATCTGGATCAGAAGGAATCTGGATGCCT
  ATTGATGACAAAGGATACTTTAATTTTGACTTCAAAACGAAACGTTTCGATGATTTAGAG
  TTAAAGAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTG
  AAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACT
  AAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTA
  CATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGAT
  AAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAATGCAGTTGTGAAGGTA
  GACTCTGAACTAGGTGAAGGTCAAGAATTCCCTGATTTGCAAGTCGATGAAAAAGGTGAA
  TTCAGCTTTGATGTAGATCATGCTGGATTTAGATTACAAAATGGAGAAACACTAAACTTC
  ACAGTAGTTGATCCTATTACAGGTGAATTATTAAGTGGAAATTTTGTTTCTAAAAACATA
  GATATTTATGAATCTCCTGAAGAAAAAGCAGATCGTGAGTTTGATGAAAGAATGGAAAAC
  ACACCTGCATATCATAAATTACATGGTGATAAAATTGTCGGCTACGATACTAACGGATTC
  CCGATTACCTGGTTTTATCCATTAGGTGAAAAGAAAGTTGAACGTAAGGCACCAAAATTA
  GAAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0401 [new locus tag: NWMN_RS02270 ]
• symbol: NWMN_0401
• description: hypothetical protein
• length: 502
• theoretical pI: 4.51591
• theoretical MW: 56546.4
• GRAVY: -0.79243

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: GQAEASEN
• SignalP: Signal peptide SP(Sec/SPI) length 32 aa
  • SP(Sec/SPI): 0.993749
  • TAT(Tat/SPI): 0.002136
  • LIPO(Sec/SPII): 0.001777
  • Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9502
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220613 NCBI
• RefSeq: YP_001331435 NCBI
• UniProt: A0A0H3K6A9 UniProt

⊟Protein sequence

• MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNINVDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTIDKKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEETSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPDLQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKADREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: set11nm > NWMN_0401

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications